Abstract
To analyze the regulatory mechanism of connective tissue growth factor expression, the 3'-untranslated region (3'-UTR) of CTGF cDNA was amplified from HeLa cell RNA. Direct nucleotide sequencing revealed a single major population in the amplicon, which was nearly identical to other sequences. Subsequently, the effect of the 3'-UTR on gene expression was evaluated. When it was fused downstream of a firefly luciferase gene, the 3'-UTR strongly repressed luciferase gene expression. Interestingly, the repressive effect of the antisense 3'-UTR appeared to be more prominent than that of the sense one. Together with the fact that several consensus sequences for regulatory elements are found in it, these results suggest the involvement of multiple sets of regulatory elements in the CTGF 3'-UTR. Copyright (C) 1999 Federation of European Biochemical Societies.
| Original language | English |
|---|---|
| Pages (from-to) | 84-88 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 450 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Apr 30 1999 |
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Keywords
- 3'-Untranslated region
- Cartilage
- Chondrocyte
- Connective tissue growth factor
- Gene expression
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Involvement of cis-acting repressive element(s) in the 3'-untranslated region of human connective tissue growth factor gene. / Kubota, Satoshi; Hattori, Takako; Nakanishi, Tohru; Takigawa, Masaharu.
In: FEBS Letters, Vol. 450, No. 1-2, 30.04.1999, p. 84-88.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Involvement of cis-acting repressive element(s) in the 3'-untranslated region of human connective tissue growth factor gene
AU - Kubota, Satoshi
AU - Hattori, Takako
AU - Nakanishi, Tohru
AU - Takigawa, Masaharu
PY - 1999/4/30
Y1 - 1999/4/30
N2 - To analyze the regulatory mechanism of connective tissue growth factor expression, the 3'-untranslated region (3'-UTR) of CTGF cDNA was amplified from HeLa cell RNA. Direct nucleotide sequencing revealed a single major population in the amplicon, which was nearly identical to other sequences. Subsequently, the effect of the 3'-UTR on gene expression was evaluated. When it was fused downstream of a firefly luciferase gene, the 3'-UTR strongly repressed luciferase gene expression. Interestingly, the repressive effect of the antisense 3'-UTR appeared to be more prominent than that of the sense one. Together with the fact that several consensus sequences for regulatory elements are found in it, these results suggest the involvement of multiple sets of regulatory elements in the CTGF 3'-UTR. Copyright (C) 1999 Federation of European Biochemical Societies.
AB - To analyze the regulatory mechanism of connective tissue growth factor expression, the 3'-untranslated region (3'-UTR) of CTGF cDNA was amplified from HeLa cell RNA. Direct nucleotide sequencing revealed a single major population in the amplicon, which was nearly identical to other sequences. Subsequently, the effect of the 3'-UTR on gene expression was evaluated. When it was fused downstream of a firefly luciferase gene, the 3'-UTR strongly repressed luciferase gene expression. Interestingly, the repressive effect of the antisense 3'-UTR appeared to be more prominent than that of the sense one. Together with the fact that several consensus sequences for regulatory elements are found in it, these results suggest the involvement of multiple sets of regulatory elements in the CTGF 3'-UTR. Copyright (C) 1999 Federation of European Biochemical Societies.
KW - 3'-Untranslated region
KW - Cartilage
KW - Chondrocyte
KW - Connective tissue growth factor
KW - Gene expression
UR - http://www.scopus.com/inward/record.url?scp=0033003978&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033003978&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(99)00480-9
DO - 10.1016/S0014-5793(99)00480-9
M3 - Article
C2 - 10350062
AN - SCOPUS:0033003978
VL - 450
SP - 84
EP - 88
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-2
ER -