Involvement of cis-acting repressive element(s) in the 3'-untranslated region of human connective tissue growth factor gene

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42 Citations (Scopus)

Abstract

To analyze the regulatory mechanism of connective tissue growth factor expression, the 3'-untranslated region (3'-UTR) of CTGF cDNA was amplified from HeLa cell RNA. Direct nucleotide sequencing revealed a single major population in the amplicon, which was nearly identical to other sequences. Subsequently, the effect of the 3'-UTR on gene expression was evaluated. When it was fused downstream of a firefly luciferase gene, the 3'-UTR strongly repressed luciferase gene expression. Interestingly, the repressive effect of the antisense 3'-UTR appeared to be more prominent than that of the sense one. Together with the fact that several consensus sequences for regulatory elements are found in it, these results suggest the involvement of multiple sets of regulatory elements in the CTGF 3'-UTR. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)84-88
Number of pages5
JournalFEBS Letters
Volume450
Issue number1-2
DOIs
Publication statusPublished - Apr 30 1999

Keywords

  • 3'-Untranslated region
  • Cartilage
  • Chondrocyte
  • Connective tissue growth factor
  • Gene expression

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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