Involvement of calmodulin-dependent protein kinases-I and -IV in long-term potentiation

Masaaki Tokuda; Bushra Y. Ahmed; Yun Fei Lu; Hideki Matsui; Osamu Miyamoto; Fuminori Yamaguchi; Ryoji Konishi; Osamu Hatase

doi:10.1016/S0006-8993(97)00189-3

Involvement of calmodulin-dependent protein kinases-I and -IV in long-term potentiation

Masaaki Tokuda, Bushra Y. Ahmed, Yun Fei Lu, Hideki Matsui, Osamu Miyamoto, Fuminori Yamaguchi, Ryoji Konishi, Osamu Hatase

Research output: Contribution to journal › Article

23 Citations (Scopus)

Abstract

Multifunctional Ca²⁺/calmodulin-dependent protein kinases (CaMKs) are thought to be involved in the induction of long-term potentiation (LTP). In the present study, LTP was induced by theta burst stimulation in the Schaffer collateral area of the stratum radiatum in the hippocampal CA1 region of the rat hippocampus. LTP-induced and control hippocampal slices were studied by Western blot and immunohistochemical analyses using CaMK-I, -II and -IV antibodies. Increased amounts of all three CaMKs were found in LTP-induced hippocampal slices as indicated by Western blot as well as by the density of their immunoreactivity. Our data clearly shows that not only CaMK-II but also CaMK-I and -IV contribute to synaptic plasticity formed in LTP.

Original language	English
Pages (from-to)	162-166
Number of pages	5
Journal	Brain Research
Volume	755
Issue number	1
DOIs	https://doi.org/10.1016/S0006-8993(97)00189-3
Publication status	Published - Apr 25 1997

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Keywords

Ca/calmodulin-dependent protein kinase
hippocampus
immunohistochemistry
long-term potentiation
Western blot

ASJC Scopus subject areas

Neuroscience(all)

Cite this

Tokuda, M., Ahmed, B. Y., Lu, Y. F., Matsui, H., Miyamoto, O., Yamaguchi, F., Konishi, R., & Hatase, O. (1997). Involvement of calmodulin-dependent protein kinases-I and -IV in long-term potentiation. Brain Research, 755(1), 162-166. https://doi.org/10.1016/S0006-8993(97)00189-3

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abstract = "Multifunctional Ca2+/calmodulin-dependent protein kinases (CaMKs) are thought to be involved in the induction of long-term potentiation (LTP). In the present study, LTP was induced by theta burst stimulation in the Schaffer collateral area of the stratum radiatum in the hippocampal CA1 region of the rat hippocampus. LTP-induced and control hippocampal slices were studied by Western blot and immunohistochemical analyses using CaMK-I, -II and -IV antibodies. Increased amounts of all three CaMKs were found in LTP-induced hippocampal slices as indicated by Western blot as well as by the density of their immunoreactivity. Our data clearly shows that not only CaMK-II but also CaMK-I and -IV contribute to synaptic plasticity formed in LTP.",

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AU - Yamaguchi, Fuminori

AU - Konishi, Ryoji

AU - Hatase, Osamu

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10.1016/S0006-8993(97)00189-3