TY - JOUR
T1 - Involvement of calmodulin-dependent protein kinases-I and -IV in long-term potentiation
AU - Tokuda, Masaaki
AU - Ahmed, Bushra Y.
AU - Lu, Yun Fei
AU - Matsui, Hideki
AU - Miyamoto, Osamu
AU - Yamaguchi, Fuminori
AU - Konishi, Ryoji
AU - Hatase, Osamu
N1 - Funding Information:
We are grateful to Dr. Angus C. Nairn (Rockefeller University, USA) and Dr. Hitoshi Fujisawa (Asahikawa University, Japan) for their generous gifts of CaMK-I and -IV antibodies, respectively. We also thank Mr. Keith Philibert (University of Calgary, Canada) for his technical assistance. This work was supported by the grant-in-aid for Scientific Research from The Ministry of Science and Technology, Government of Pakistan and from The Ministry of Education, Science and Culture of Japan.
PY - 1997/4/25
Y1 - 1997/4/25
N2 - Multifunctional Ca2+/calmodulin-dependent protein kinases (CaMKs) are thought to be involved in the induction of long-term potentiation (LTP). In the present study, LTP was induced by theta burst stimulation in the Schaffer collateral area of the stratum radiatum in the hippocampal CA1 region of the rat hippocampus. LTP-induced and control hippocampal slices were studied by Western blot and immunohistochemical analyses using CaMK-I, -II and -IV antibodies. Increased amounts of all three CaMKs were found in LTP-induced hippocampal slices as indicated by Western blot as well as by the density of their immunoreactivity. Our data clearly shows that not only CaMK-II but also CaMK-I and -IV contribute to synaptic plasticity formed in LTP.
AB - Multifunctional Ca2+/calmodulin-dependent protein kinases (CaMKs) are thought to be involved in the induction of long-term potentiation (LTP). In the present study, LTP was induced by theta burst stimulation in the Schaffer collateral area of the stratum radiatum in the hippocampal CA1 region of the rat hippocampus. LTP-induced and control hippocampal slices were studied by Western blot and immunohistochemical analyses using CaMK-I, -II and -IV antibodies. Increased amounts of all three CaMKs were found in LTP-induced hippocampal slices as indicated by Western blot as well as by the density of their immunoreactivity. Our data clearly shows that not only CaMK-II but also CaMK-I and -IV contribute to synaptic plasticity formed in LTP.
KW - Ca/calmodulin-dependent protein kinase
KW - Western blot
KW - hippocampus
KW - immunohistochemistry
KW - long-term potentiation
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U2 - 10.1016/S0006-8993(97)00189-3
DO - 10.1016/S0006-8993(97)00189-3
M3 - Article
C2 - 9163554
AN - SCOPUS:0030894369
VL - 755
SP - 162
EP - 166
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1
ER -