Involvement of arachidonic acid in nonimmunologic production of superoxide in mast cells

Masakiyo Sakaguchi, Nobuyuki Fukuishi, Kazuyo Teramoto, Masahiro Miyazaki, Nam Ho Huh, Masayoshi Namba, Masaaki Akagi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: A number of different molecules are known to be involved in the signal pathway to release histamine from mast cells, among which arachidonic acid (AA) is one of the key mediators. On the other hand, we found that the application of compound 48/80, a typical histamine liberator, generated superoxide in mast cells. In the present study, we investigated the mechanism of superoxide production in mast cells with respect to AA signaling in conjunction with a fine structural analysis. Methods: Superoxide production was monitored by chemiluminescence in rat peritoneal mast cells and their subfractions after various treatments. For scanning electron micrography, the conditions for fixation and freeze-fracture were optimized to get natural fine images. Results: Compound 48/80 induced superoxide production in the isolated mast cells and some of their subfractions possibly through intracellular increase in Ca2+ concentration, activation of cytosolic phospholipase A2, and release of AA. Discussion: The present results indicate the critical role of AA in the signal pathway to generate superoxide from mast cells in response to compound 48/80.

Original languageEnglish
Pages (from-to)288-299
Number of pages12
JournalInternational archives of allergy and immunology
Volume130
Issue number4
DOIs
Publication statusPublished - 2003

Keywords

  • Arachidonic acid
  • Mast cells
  • Phospholipase A
  • Superoxide

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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