Involvement of annexin-I in glucose-induced insulin secretion in rat pancreatic islets

M. Ohnishi, M. Tokuda, T. Masaki, T. Fujimura, Y. Tai, T. Itano, Hideki Matsui, T. Ishida, R. Konishi, J. Takahara, O. Hatase

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Abstract

Annexin-I was demonstrated to specifically present in islets and not in exocrine tissues of the rat pancreas and to have a diffuse and homogeneous distribution in all islet cells in our previous study. In the present report, to clarify the functions of annexin-I in rat pancreatic islets, especially in β-cells, we investigated the role of annexin-I in insulin secretion. Immunoelectron microscopic analysis of pancreatic β-cells demonstrated that immunogold particles reactive to annexin-I were almost exclusively observed on most of the insulin-containing granules (~90%) and less frequently located in cytosol and other organelles, such as the endoplasmic reticulum and mitochondria. The number of annexin-I gold particles located on insulin granules after oral glucose administration was significantly increased compared with that observed in fasted rats. Moreover, when the isolated islets were stimulated by a high concentration of glucose (20 mM), the phosphorylation of annexin-I was markedly enhanced, and it was synchronized to insulin secretion. This phosphorylation mainly occurred on serine residues. H-7 (100 μM), a potent inhibitor of protein kinase-C, inhibited the phosphorylation to about 90%. These findings suggest that annexin-I might be involved in the regulatory mechanism of glucose-induced insulin secretion in rat pancreatic islets via phosphorylation-dephosphorylation processes.

Original languageEnglish
Pages (from-to)2421-2426
Number of pages6
JournalEndocrinology
Volume136
Issue number6
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Annexin A1
Islets of Langerhans
Insulin
Glucose
Phosphorylation
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Gold
Endoplasmic Reticulum
Organelles
Cytosol
Serine
Protein Kinase C
Oral Administration
Pancreas
Mitochondria

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Ohnishi, M., Tokuda, M., Masaki, T., Fujimura, T., Tai, Y., Itano, T., ... Hatase, O. (1995). Involvement of annexin-I in glucose-induced insulin secretion in rat pancreatic islets. Endocrinology, 136(6), 2421-2426.

Involvement of annexin-I in glucose-induced insulin secretion in rat pancreatic islets. / Ohnishi, M.; Tokuda, M.; Masaki, T.; Fujimura, T.; Tai, Y.; Itano, T.; Matsui, Hideki; Ishida, T.; Konishi, R.; Takahara, J.; Hatase, O.

In: Endocrinology, Vol. 136, No. 6, 1995, p. 2421-2426.

Research output: Contribution to journalArticle

Ohnishi, M, Tokuda, M, Masaki, T, Fujimura, T, Tai, Y, Itano, T, Matsui, H, Ishida, T, Konishi, R, Takahara, J & Hatase, O 1995, 'Involvement of annexin-I in glucose-induced insulin secretion in rat pancreatic islets', Endocrinology, vol. 136, no. 6, pp. 2421-2426.
Ohnishi M, Tokuda M, Masaki T, Fujimura T, Tai Y, Itano T et al. Involvement of annexin-I in glucose-induced insulin secretion in rat pancreatic islets. Endocrinology. 1995;136(6):2421-2426.
Ohnishi, M. ; Tokuda, M. ; Masaki, T. ; Fujimura, T. ; Tai, Y. ; Itano, T. ; Matsui, Hideki ; Ishida, T. ; Konishi, R. ; Takahara, J. ; Hatase, O. / Involvement of annexin-I in glucose-induced insulin secretion in rat pancreatic islets. In: Endocrinology. 1995 ; Vol. 136, No. 6. pp. 2421-2426.
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AU - Tai, Y.

AU - Itano, T.

AU - Matsui, Hideki

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AU - Takahara, J.

AU - Hatase, O.

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