TY - JOUR
T1 - Involvement of a periodontal pathogen, Porphyromonas gingivalis on the pathogenesis of non-alcoholic fatty liver disease
AU - Yoneda, Masato
AU - Naka, Shuhei
AU - Nakano, Kazuhiko
AU - Wada, Koichiro
AU - Endo, Hiroki
AU - Mawatari, Hironori
AU - Imajo, Kento
AU - Nomura, Ryota
AU - Hokamura, Kazuya
AU - Ono, Masafumi
AU - Murata, Shogo
AU - Tohnai, Iwai
AU - Sumida, Yoshio
AU - Shima, Toshihide
AU - Kuboniwa, Masae
AU - Umemura, Kazuo
AU - Kamisaki, Yoshinori
AU - Amano, Atsuo
AU - Okanoue, Takeshi
AU - Ooshima, Takashi
AU - Nakajima, Atsushi
N1 - Funding Information:
This study was supported by a Grant-in-Aid from the Ministry of Health, Labour and Welfare, Japan, to A. N., a grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan to T.O. (Grant-in-Aid for Scientific Research (A)), K.N. (Grants-in-Aid for Young Scientists (A)), R.N. (Grants-in-Aid for Young Scientists (B)), and M.Y., Y.A., K.W., A. N. (Challenging Exploratory Research), and a grant program, “A-STAGE,” from the Japan Science and Technology Agency (JST) to A.N.
PY - 2012/2/16
Y1 - 2012/2/16
N2 - Background: Non-alcoholic fatty liver disease (NAFLD) is a hepatic manifestation of metabolic syndrome that is closely associated with multiple factors such as obesity, hyperlipidemia and type 2 diabetes mellitus. However, other risk factors for the development of NAFLD are unclear. With the association between periodontal disease and the development of systemic diseases receiving increasing attention recently, we conducted this study to investigate the relationship between NAFLD and infection with Porphyromonas gingivalis (P. gingivalis), a major causative agent of periodontitis.Methods: The detection frequencies of periodontal bacteria in oral samples collected from 150 biopsy-proven NAFLD patients (102 with non-alcoholic steatohepatitis (NASH) and 48 with non-alcoholic fatty liver (NAFL) patients) and 60 non-NAFLD control subjects were determined. Detection of P. gingivalis and other periodontopathic bacteria were detected by PCR assay. In addition, effect of P. gingivalis-infection on mouse NAFLD model was investigated. To clarify the exact contribution of P. gingivalis-induced periodontitis, non-surgical periodontal treatments were also undertaken for 3 months in 10 NAFLD patients with periodontitis.Results: The detection frequency of P. gingivalis in NAFLD patients was significantly higher than that in the non-NAFLD control subjects (46.7% vs. 21.7%, odds ratio: 3.16). In addition, the detection frequency of P. gingivalis in NASH patients was markedly higher than that in the non-NAFLD subjects (52.0%, odds ratio: 3.91). Most of the P. gingivalis fimbria detected in the NAFLD patients was of invasive genotypes, especially type II (50.0%). Infection of type II P. gingivalis on NAFLD model of mice accelerated the NAFLD progression. The non-surgical periodontal treatments on NAFLD patients carried out for 3 months ameliorated the liver function parameters, such as the serum levels of AST and ALT.Conclusions: Infection with high-virulence P. gingivalis might be an additional risk factor for the development/progression of NAFLD/NASH.
AB - Background: Non-alcoholic fatty liver disease (NAFLD) is a hepatic manifestation of metabolic syndrome that is closely associated with multiple factors such as obesity, hyperlipidemia and type 2 diabetes mellitus. However, other risk factors for the development of NAFLD are unclear. With the association between periodontal disease and the development of systemic diseases receiving increasing attention recently, we conducted this study to investigate the relationship between NAFLD and infection with Porphyromonas gingivalis (P. gingivalis), a major causative agent of periodontitis.Methods: The detection frequencies of periodontal bacteria in oral samples collected from 150 biopsy-proven NAFLD patients (102 with non-alcoholic steatohepatitis (NASH) and 48 with non-alcoholic fatty liver (NAFL) patients) and 60 non-NAFLD control subjects were determined. Detection of P. gingivalis and other periodontopathic bacteria were detected by PCR assay. In addition, effect of P. gingivalis-infection on mouse NAFLD model was investigated. To clarify the exact contribution of P. gingivalis-induced periodontitis, non-surgical periodontal treatments were also undertaken for 3 months in 10 NAFLD patients with periodontitis.Results: The detection frequency of P. gingivalis in NAFLD patients was significantly higher than that in the non-NAFLD control subjects (46.7% vs. 21.7%, odds ratio: 3.16). In addition, the detection frequency of P. gingivalis in NASH patients was markedly higher than that in the non-NAFLD subjects (52.0%, odds ratio: 3.91). Most of the P. gingivalis fimbria detected in the NAFLD patients was of invasive genotypes, especially type II (50.0%). Infection of type II P. gingivalis on NAFLD model of mice accelerated the NAFLD progression. The non-surgical periodontal treatments on NAFLD patients carried out for 3 months ameliorated the liver function parameters, such as the serum levels of AST and ALT.Conclusions: Infection with high-virulence P. gingivalis might be an additional risk factor for the development/progression of NAFLD/NASH.
KW - Insulin resistance
KW - Non-alcoholic fatty liver disease (NAFLD)
KW - Non-alcoholic steatohepatitis (NASH)
KW - Oral bacteria
KW - P. gingivalis
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U2 - 10.1186/1471-230X-12-16
DO - 10.1186/1471-230X-12-16
M3 - Article
C2 - 22340817
AN - SCOPUS:84856836048
SN - 1471-230X
VL - 12
JO - BMC Gastroenterology
JF - BMC Gastroenterology
M1 - 16
ER -
