Involvement of a non-proton pump factor (possibly Donnan-type equilibrium) in maintenance of an acidic pH in lysosomes

Yoshinori Moriyama, Masatomo Maeda, Masamitsu Futai

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Change of the internal pH of isolated lysosomes was measured with fluorescein isothiocyanate-dextran. In buffer of pH 7.0, isolated lysosomes had an acidic pH of about 5.5, which decreased to pH 5.2 on addition of ATP. Addition of bafilomycin inhibited the acidification by H+-ATPase and resulted in an increase of the internal pH to 5.5 due to passive diffusion of protons across the lysosomal membrane. However, no further alkalization was observed. The acidic pH (pH 5.5) of isolated lysosomes could be maintained for at least 48 h in the absence of ATP, but increased gradually to pH 5.9-6.4 upon incubation with monovalent cations (K+ or Na+), amines, or ionophores. These results suggest that a non-proton pump factor (possibly Donnan equilibrium) is involved in maintaining the acidic pH of isolated lysosomes.

Original languageEnglish
Pages (from-to)18-20
Number of pages3
JournalFEBS Letters
Volume302
Issue number1
DOIs
Publication statusPublished - May 4 1992
Externally publishedYes

Fingerprint

Lysosomes
Adenosine Triphosphate
Maintenance
Pumps
Monovalent Cations
Proton-Translocating ATPases
Acidification
Ionophores
Amines
Protons
Buffers
Membranes
fluorescein isothiocyanate dextran

Keywords

  • Acidic pH
  • Donnan equilibrium
  • Lysosome
  • Proton pump

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Involvement of a non-proton pump factor (possibly Donnan-type equilibrium) in maintenance of an acidic pH in lysosomes. / Moriyama, Yoshinori; Maeda, Masatomo; Futai, Masamitsu.

In: FEBS Letters, Vol. 302, No. 1, 04.05.1992, p. 18-20.

Research output: Contribution to journalArticle

Moriyama, Yoshinori ; Maeda, Masatomo ; Futai, Masamitsu. / Involvement of a non-proton pump factor (possibly Donnan-type equilibrium) in maintenance of an acidic pH in lysosomes. In: FEBS Letters. 1992 ; Vol. 302, No. 1. pp. 18-20.
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