Investigation of the renal injury caused by liver ischemia-reperfusion in rats

Yoshikuni Kudo, Toru Egashira, Fusako Takayama, Yasumitsu Yamanaka, Tatsuo Shimada

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

To explain the mechanism of renal injury caused by liver ischemia-reperfusion, we investigated biochemical and morphological changes in the liver and kidney in rats. After reperfusion following 60 min of liver ischemia, numerous changes were found. The level of serum transaminases and lipid peroxide formation in the liver tissue increased significantly. Electron microscopic studies revealed that most of the hepatocytes had swollen mitochondria and clumping of the nuclear chromatin. The sinusoidal endothelium was disrupted and the sinusoidal lumen was filled with numerous erythrocytes. Blood endotoxin concentration, plasma lipid peroxide levels, and serum β-glucuronidase activities were significantly higher than in the control group. Biochemical and morphological renal injury was also observed. Tissue lipid peroxide levels increased in both the kidney and the liver. Microscopic examination revealed damage to the renal tubules, including interstitial edema, dilatation of the lumen, and granular casts derived from necrotic cells in the proximal convoluted tubule. The levels of urinary N-acetyl-β-d-glucosaminidase (NAG) in the liver ischemia-reperfusion group were also higher than in the control group. These results suggest that the renal injury was caused by an increase in endotoxin, lipid peroxide, and lysosomal enzymes in the blood following the liver injury induced by the ischemia-reperfusion.

Original languageEnglish
Pages (from-to)502-509
Number of pages8
JournalArchives of Toxicology
Volume67
Issue number7
DOIs
Publication statusPublished - Aug 1993
Externally publishedYes

Fingerprint

Liver
Reperfusion
Rats
Ischemia
Kidney
Lipid Peroxides
Wounds and Injuries
Endotoxins
Blood
Tissue
Hexosaminidases
Control Groups
Mitochondria
Glucuronidase
Transaminases
Reperfusion Injury
Serum
Chromatin
Endothelium
Dilatation

Keywords

  • Endotoxin
  • Ischemia-reperfusion
  • Kidney
  • Lipid peroxidation
  • Liver

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Investigation of the renal injury caused by liver ischemia-reperfusion in rats. / Kudo, Yoshikuni; Egashira, Toru; Takayama, Fusako; Yamanaka, Yasumitsu; Shimada, Tatsuo.

In: Archives of Toxicology, Vol. 67, No. 7, 08.1993, p. 502-509.

Research output: Contribution to journalArticle

Kudo, Yoshikuni ; Egashira, Toru ; Takayama, Fusako ; Yamanaka, Yasumitsu ; Shimada, Tatsuo. / Investigation of the renal injury caused by liver ischemia-reperfusion in rats. In: Archives of Toxicology. 1993 ; Vol. 67, No. 7. pp. 502-509.
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