TY - GEN
T1 - Investigation of multilayered protein adsorption on carbonate apatite with a QCM technique
AU - Yoshioka, Tomohiko
AU - Yonekura, Hiroshi
AU - Ikoma, Toshiyuki
AU - Tagaya, Motohiro
AU - Tanaka, Junzo
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2013
Y1 - 2013
N2 - This study is concerned with multilayered protein adsorption on carbonate apatite (CAp) that is measured with a quartz crystal microbalance (QCM) technique. A carbonate apatite-deposited QCM sensor was prepared by electrophoretic deposition (EPD). On the CAp-deposited QCM sensor, fibrinogen adsorption followed by thrombin adsorption was investigated. The adsorption of fibrinogen on CAp led to a clear decrease in the resonance frequency of the sensor, which means that fibrinogen adsorbed on the CAp-deposited QCM sensor. The adsorbed amount of fibrinogen was larger on CAp than on a pristine sensor (Au). Although successive thrombin adsorption occurred on fibrinogen adlayers on both CAp and Au, the adsorbed amount and its conformation were different depending on the surface.
AB - This study is concerned with multilayered protein adsorption on carbonate apatite (CAp) that is measured with a quartz crystal microbalance (QCM) technique. A carbonate apatite-deposited QCM sensor was prepared by electrophoretic deposition (EPD). On the CAp-deposited QCM sensor, fibrinogen adsorption followed by thrombin adsorption was investigated. The adsorption of fibrinogen on CAp led to a clear decrease in the resonance frequency of the sensor, which means that fibrinogen adsorbed on the CAp-deposited QCM sensor. The adsorbed amount of fibrinogen was larger on CAp than on a pristine sensor (Au). Although successive thrombin adsorption occurred on fibrinogen adlayers on both CAp and Au, the adsorbed amount and its conformation were different depending on the surface.
KW - Carbonate apatite
KW - Protein adsorption
KW - Quartz crystal microbalance (QCM)
UR - http://www.scopus.com/inward/record.url?scp=84871289170&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84871289170&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/KEM.529-530.74
DO - 10.4028/www.scientific.net/KEM.529-530.74
M3 - Conference contribution
AN - SCOPUS:84871289170
SN - 9783037855171
T3 - Key Engineering Materials
SP - 74
EP - 77
BT - Bioceramics 24
PB - Trans Tech Publications Ltd
T2 - 24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012
Y2 - 21 October 2012 through 24 October 2012
ER -