Investigation of multilayered protein adsorption on carbonate apatite with a QCM technique

Tomohiko Yoshioka; Hiroshi Yonekura; Toshiyuki Ikoma; Motohiro Tagaya; Junzo Tanaka

doi:10.4028/www.scientific.net/KEM.529-530.74

Investigation of multilayered protein adsorption on carbonate apatite with a QCM technique

Tomohiko Yoshioka, Hiroshi Yonekura, Toshiyuki Ikoma, Motohiro Tagaya, Junzo Tanaka

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

This study is concerned with multilayered protein adsorption on carbonate apatite (CAp) that is measured with a quartz crystal microbalance (QCM) technique. A carbonate apatite-deposited QCM sensor was prepared by electrophoretic deposition (EPD). On the CAp-deposited QCM sensor, fibrinogen adsorption followed by thrombin adsorption was investigated. The adsorption of fibrinogen on CAp led to a clear decrease in the resonance frequency of the sensor, which means that fibrinogen adsorbed on the CAp-deposited QCM sensor. The adsorbed amount of fibrinogen was larger on CAp than on a pristine sensor (Au). Although successive thrombin adsorption occurred on fibrinogen adlayers on both CAp and Au, the adsorbed amount and its conformation were different depending on the surface.

Original language	English
Title of host publication	Bioceramics 24
Publisher	Trans Tech Publications Ltd
Pages	74-77
Number of pages	4
Edition	1
ISBN (Print)	9783037855171
DOIs	https://doi.org/10.4028/www.scientific.net/KEM.529-530.74
Publication status	Published - 2013
Externally published	Yes
Event	24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012 - Fukuoka, Japan Duration: Oct 21 2012 → Oct 24 2012

Publication series

Name	Key Engineering Materials
Number	1
Volume	529-530
ISSN (Print)	1013-9826
ISSN (Electronic)	1662-9795

Other

Other	24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012
Country	Japan
City	Fukuoka
Period	10/21/12 → 10/24/12

Keywords

Carbonate apatite
Protein adsorption
Quartz crystal microbalance (QCM)

ASJC Scopus subject areas

Materials Science(all)
Mechanics of Materials
Mechanical Engineering

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Investigation of multilayered protein adsorption on carbonate apatite with a QCM technique. / Yoshioka, Tomohiko; Yonekura, Hiroshi; Ikoma, Toshiyuki; Tagaya, Motohiro; Tanaka, Junzo.

Bioceramics 24. 1. ed. Trans Tech Publications Ltd, 2013. p. 74-77 (Key Engineering Materials; Vol. 529-530, No. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Yoshioka, T, Yonekura, H, Ikoma, T, Tagaya, M & Tanaka, J 2013, Investigation of multilayered protein adsorption on carbonate apatite with a QCM technique. in Bioceramics 24. 1 edn, Key Engineering Materials, no. 1, vol. 529-530, Trans Tech Publications Ltd, pp. 74-77, 24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012, Fukuoka, Japan, 10/21/12. https://doi.org/10.4028/www.scientific.net/KEM.529-530.74

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abstract = "This study is concerned with multilayered protein adsorption on carbonate apatite (CAp) that is measured with a quartz crystal microbalance (QCM) technique. A carbonate apatite-deposited QCM sensor was prepared by electrophoretic deposition (EPD). On the CAp-deposited QCM sensor, fibrinogen adsorption followed by thrombin adsorption was investigated. The adsorption of fibrinogen on CAp led to a clear decrease in the resonance frequency of the sensor, which means that fibrinogen adsorbed on the CAp-deposited QCM sensor. The adsorbed amount of fibrinogen was larger on CAp than on a pristine sensor (Au). Although successive thrombin adsorption occurred on fibrinogen adlayers on both CAp and Au, the adsorbed amount and its conformation were different depending on the surface.",

keywords = "Carbonate apatite, Protein adsorption, Quartz crystal microbalance (QCM)",

author = "Tomohiko Yoshioka and Hiroshi Yonekura and Toshiyuki Ikoma and Motohiro Tagaya and Junzo Tanaka",

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AB - This study is concerned with multilayered protein adsorption on carbonate apatite (CAp) that is measured with a quartz crystal microbalance (QCM) technique. A carbonate apatite-deposited QCM sensor was prepared by electrophoretic deposition (EPD). On the CAp-deposited QCM sensor, fibrinogen adsorption followed by thrombin adsorption was investigated. The adsorption of fibrinogen on CAp led to a clear decrease in the resonance frequency of the sensor, which means that fibrinogen adsorbed on the CAp-deposited QCM sensor. The adsorbed amount of fibrinogen was larger on CAp than on a pristine sensor (Au). Although successive thrombin adsorption occurred on fibrinogen adlayers on both CAp and Au, the adsorbed amount and its conformation were different depending on the surface.

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