Investigation for presence of Neospora caninum, Toxoplasma gondii and Brucella-species infection in killer whales (Orcinus orca) mass-stranded on the coast of Shiretoko, Hokkaido, Japan

Yoshitaka Omata, Yusuke Umeshita, Masahisa Watarai, Masato Tachibana, Motoki Sasaki, Koichi Murata, Tadasu K. Yamada

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Twelve killer whale (Orcinus orca) were hemmed in by ice floes, and nine died on the Aidomari coast in the Nemuro Strait in Rausu, Shiretoko, Hokkaido, Japan on 8 February 2005. Tissue samples collected from 8 whales were tested for Neospora caninum, Toxoplasma gondii, and Brucella species DNA by polymerase chain reaction (PCR) assay. Gamma-globulin isolated from blood samples by ammonium sulfate precipitation was tested for antibodies to these pathogens by means of agglutination tests and immunoblotting. None of the 8 tissue samples had antibodies to the pathogens, when subjected to agglutination tests. In immunoblotting, one sample (sample No.5) showed antibody binding to N. caninum antigens. In the PCR assay, none of the samples was positive. Further study is necessary to examine the prevalence of the pathogens in marine mammals inhabiting this area.

Original languageEnglish
Pages (from-to)523-526
Number of pages4
JournalJournal of Veterinary Medical Science
Volume68
Issue number5
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Killer Whale
Neospora
Orcinus orca
Brucella
Neospora caninum
Toxoplasma
Toxoplasma gondii
Japan
Agglutination Tests
Immunoblotting
coasts
Antibodies
Infection
infection
Whales
Polymerase Chain Reaction
gamma-Globulins
agglutination tests
Ammonium Sulfate
Ice

Keywords

  • Infection
  • Killer whale
  • Mass-stranding

ASJC Scopus subject areas

  • veterinary(all)
  • Marketing

Cite this

Investigation for presence of Neospora caninum, Toxoplasma gondii and Brucella-species infection in killer whales (Orcinus orca) mass-stranded on the coast of Shiretoko, Hokkaido, Japan. / Omata, Yoshitaka; Umeshita, Yusuke; Watarai, Masahisa; Tachibana, Masato; Sasaki, Motoki; Murata, Koichi; Yamada, Tadasu K.

In: Journal of Veterinary Medical Science, Vol. 68, No. 5, 2006, p. 523-526.

Research output: Contribution to journalArticle

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