Inverse correlation between mRNA levels in GFP-silenced transgenic Nicotiana benthamiana and resistance to Potato virus X engineered to contain GFP sequence

Masaki Shimono, Makoto Ino, Shoji Sonoda, Tatsuhito Fujimura, Masamichi Nishiguchi

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2 Citations (Scopus)

Abstract

Nicotiana benthamiana was transformed with a green fluorescent protein (GFP) gene driven by cauliflower mosaic virus 35S promoter. A GFP-silenced line and a nonsilenced line were selected after ultraviolet irradiation. GFP short-interfering RNAs (siRNAs) were detected in the silenced line but not in the nonsilenced line. T1 progeny of the silenced line varied in GFP suppression patterns and were grouped into three types (I, II, III) based on the GFP suppression pattern. With Northern blot analysis, different levels of GFP mRNA accumulated, from a very low level in type I and II to an intermediate level in type III, in contrast to a much higher level in the nonsilenced line. Plants were also inoculated with Potato virus X engineered to contain the GFP sequence to evaluate the levels of virus resistance. None to a few GFP spots were observed on inoculated leaves in types I and II, whereas numerous spots and systemic infection appeared in type III. These results showed that virus resistance was inversely correlated with the levels of mRNA, suggesting that the strength of RNA silencing determines the extent of virus resistance.

Original languageEnglish
Pages (from-to)147-152
Number of pages6
JournalJournal of General Plant Pathology
Volume71
Issue number2
DOIs
Publication statusPublished - Apr 1 2005

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Keywords

  • Green fluorescent protein
  • Nicotiana benthamiana
  • Posttranscriptional gene silencing
  • Transgenic plant
  • Virus resistance

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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