Introduction of the rolC gene into the genome of the Japanese persimmon causes dwarfism

Yoshiko Koshita, Yuri Nakamura, Shozo Kobayashi, Kunihisa Morinaga

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The rolC gene from Agrobacterium rhizogenes was introduced into a seedling of the Japanese persimmon (Diospyros kaki L.) 'Saijo' genome by Agrobacterium-mediated transformation. Integration of the rolC gene into the genome was confirmed by Southern blot analysis. Northern blot analysis revealed that the transgene was transcribed in the transgenic persimmon. The transformed plant (PC1) had shorter internodes and smaller leaves and produced more branches than the regenerated plants from the open-pollinated 'Saijo' seedlings. These results show the potential use of the rolC gene to produce dwarf Japanese persimmon plants.

Original languageEnglish
Pages (from-to)529-531
Number of pages3
JournalJournal of the Japanese Society for Horticultural Science
Volume71
Issue number4
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

persimmons
dwarfing
genome
Diospyros kaki
Rhizobium rhizogenes
genes
seedlings
Agrobacterium
internodes
Northern blotting
branches
Southern blotting
transgenes
genetically modified organisms
leaves

Keywords

  • Dwarfism
  • Persimmon
  • rolC gene
  • Transformation

ASJC Scopus subject areas

  • Horticulture
  • Plant Science

Cite this

Introduction of the rolC gene into the genome of the Japanese persimmon causes dwarfism. / Koshita, Yoshiko; Nakamura, Yuri; Kobayashi, Shozo; Morinaga, Kunihisa.

In: Journal of the Japanese Society for Horticultural Science, Vol. 71, No. 4, 2002, p. 529-531.

Research output: Contribution to journalArticle

Koshita, Yoshiko ; Nakamura, Yuri ; Kobayashi, Shozo ; Morinaga, Kunihisa. / Introduction of the rolC gene into the genome of the Japanese persimmon causes dwarfism. In: Journal of the Japanese Society for Horticultural Science. 2002 ; Vol. 71, No. 4. pp. 529-531.
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