Introduction and transposition of the maize transposable element Ac in rice (Oryza sativa L.)

Takeshi Izawa, Chikara Miyazaki, Mikihiro Yamamoto, Rie Terada, Shigeru Iida, Ko Shimamoto

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

To develop a transposon tagging system in an important cereal plant, rice (Oryza sativa L.), the maize transposable element Ac (Activator) was introduced into rice protoplasts by electroporation. We employed a phenotypic assay for excision of Ac from the selectable hph gene encoding resistance to hygromycin B. Southern blot analysis of hygromycin B-resistant calli showed that the Ac element can transpose from the introduced hph gene into the rice chromosomes. Sequence analysis of several Ac excision sites in the hph gene revealed sequence alterations characteristic of the excision sites of this plant transposable element. The Ac element appears to be active during development of transgenic rice plants from calli. Moreover, hybridization patterns of different leaves from the same plant indicated that some Ac elements are stable whereas others are able to transpose further during development of leaves. The results indicate that the introduced Ac element can transpose efficiently in transgenic rice plants.

Original languageEnglish
Pages (from-to)391-396
Number of pages6
JournalMGG Molecular & General Genetics
Volume227
Issue number3
DOIs
Publication statusPublished - Jul 1991
Externally publishedYes

Fingerprint

DNA Transposable Elements
Zea mays
Hygromycin B
Genetically Modified Plants
Bony Callus
Genes
Plant Leaves
Electroporation
Protoplasts
Southern Blotting
Sequence Analysis
Oryza
Chromosomes

Keywords

  • Imprecise excision
  • Transgenic rice plants
  • Transposable element Ac
  • Transposition
  • Transposon tagging

ASJC Scopus subject areas

  • Genetics

Cite this

Introduction and transposition of the maize transposable element Ac in rice (Oryza sativa L.). / Izawa, Takeshi; Miyazaki, Chikara; Yamamoto, Mikihiro; Terada, Rie; Iida, Shigeru; Shimamoto, Ko.

In: MGG Molecular & General Genetics, Vol. 227, No. 3, 07.1991, p. 391-396.

Research output: Contribution to journalArticle

Izawa, Takeshi ; Miyazaki, Chikara ; Yamamoto, Mikihiro ; Terada, Rie ; Iida, Shigeru ; Shimamoto, Ko. / Introduction and transposition of the maize transposable element Ac in rice (Oryza sativa L.). In: MGG Molecular & General Genetics. 1991 ; Vol. 227, No. 3. pp. 391-396.
@article{5f7e4626384f41d780508dd64bd90286,
title = "Introduction and transposition of the maize transposable element Ac in rice (Oryza sativa L.)",
abstract = "To develop a transposon tagging system in an important cereal plant, rice (Oryza sativa L.), the maize transposable element Ac (Activator) was introduced into rice protoplasts by electroporation. We employed a phenotypic assay for excision of Ac from the selectable hph gene encoding resistance to hygromycin B. Southern blot analysis of hygromycin B-resistant calli showed that the Ac element can transpose from the introduced hph gene into the rice chromosomes. Sequence analysis of several Ac excision sites in the hph gene revealed sequence alterations characteristic of the excision sites of this plant transposable element. The Ac element appears to be active during development of transgenic rice plants from calli. Moreover, hybridization patterns of different leaves from the same plant indicated that some Ac elements are stable whereas others are able to transpose further during development of leaves. The results indicate that the introduced Ac element can transpose efficiently in transgenic rice plants.",
keywords = "Imprecise excision, Transgenic rice plants, Transposable element Ac, Transposition, Transposon tagging",
author = "Takeshi Izawa and Chikara Miyazaki and Mikihiro Yamamoto and Rie Terada and Shigeru Iida and Ko Shimamoto",
year = "1991",
month = "7",
doi = "10.1007/BF00273928",
language = "English",
volume = "227",
pages = "391--396",
journal = "Molecular Genetics and Genomics",
issn = "1617-4615",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Introduction and transposition of the maize transposable element Ac in rice (Oryza sativa L.)

AU - Izawa, Takeshi

AU - Miyazaki, Chikara

AU - Yamamoto, Mikihiro

AU - Terada, Rie

AU - Iida, Shigeru

AU - Shimamoto, Ko

PY - 1991/7

Y1 - 1991/7

N2 - To develop a transposon tagging system in an important cereal plant, rice (Oryza sativa L.), the maize transposable element Ac (Activator) was introduced into rice protoplasts by electroporation. We employed a phenotypic assay for excision of Ac from the selectable hph gene encoding resistance to hygromycin B. Southern blot analysis of hygromycin B-resistant calli showed that the Ac element can transpose from the introduced hph gene into the rice chromosomes. Sequence analysis of several Ac excision sites in the hph gene revealed sequence alterations characteristic of the excision sites of this plant transposable element. The Ac element appears to be active during development of transgenic rice plants from calli. Moreover, hybridization patterns of different leaves from the same plant indicated that some Ac elements are stable whereas others are able to transpose further during development of leaves. The results indicate that the introduced Ac element can transpose efficiently in transgenic rice plants.

AB - To develop a transposon tagging system in an important cereal plant, rice (Oryza sativa L.), the maize transposable element Ac (Activator) was introduced into rice protoplasts by electroporation. We employed a phenotypic assay for excision of Ac from the selectable hph gene encoding resistance to hygromycin B. Southern blot analysis of hygromycin B-resistant calli showed that the Ac element can transpose from the introduced hph gene into the rice chromosomes. Sequence analysis of several Ac excision sites in the hph gene revealed sequence alterations characteristic of the excision sites of this plant transposable element. The Ac element appears to be active during development of transgenic rice plants from calli. Moreover, hybridization patterns of different leaves from the same plant indicated that some Ac elements are stable whereas others are able to transpose further during development of leaves. The results indicate that the introduced Ac element can transpose efficiently in transgenic rice plants.

KW - Imprecise excision

KW - Transgenic rice plants

KW - Transposable element Ac

KW - Transposition

KW - Transposon tagging

UR - http://www.scopus.com/inward/record.url?scp=0025864167&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025864167&partnerID=8YFLogxK

U2 - 10.1007/BF00273928

DO - 10.1007/BF00273928

M3 - Article

C2 - 1650907

AN - SCOPUS:0025864167

VL - 227

SP - 391

EP - 396

JO - Molecular Genetics and Genomics

JF - Molecular Genetics and Genomics

SN - 1617-4615

IS - 3

ER -