Intravenous grafts of amniotic fluid-derived stem cells induce endogenous cell proliferation and attenuate behavioral deficits in ischemic stroke rats

Naoki Tajiri, Sandra Acosta, Loren E. Glover, Paula C. Bickford, Alejandra Jacotte Simancas, Takao Yasuhara, Isao Date, Marianna A. Solomita, Ivana Antonucci, Liborio Stuppia, Yuji Kaneko, Cesar V. Borlongan

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We recently reported isolation of viable rat amniotic fluid-derived stem (AFS) cells [1]. Here, we tested the therapeutic benefits of AFS cells in a rodent model of ischemic stroke. Adult male Sprague-Dawley rats received a 60-minute middle cerebral artery occlusion (MCAo). Thirty-five days later, animals exhibiting significant motor deficits received intravenous transplants of rat AFS cells or vehicle. At days 60-63 post-MCAo, significant recovery of motor and cognitive function was seen in stroke animals transplanted with AFS cells compared to vehicle-infused stroke animals. Infarct volume, as revealed by hematoxylin and eosin (H&E) staining, was significantly reduced, coupled with significant increments in the cell proliferation marker, Ki67, and the neuronal marker, MAP2, in the dentate gyrus (DG) [2] and the subventricular zone (SVZ) of AFS cell-transplanted stroke animals compared to vehicle-infused stroke animals. A significantly higher number of double-labeled Ki67/MAP2-positive cells and a similar trend towards increased Ki67/MAP2 double-labeling were observed in the DG and SVZ of AFS cell-transplanted stroke animals, respectively, compared to vehicle-infused stroke animals. This study reports the therapeutic potential of AFS cell transplantation in stroke animals, possibly via enhancement of endogenous repair mechanisms.

Original languageEnglish
Article numbere43779
JournalPLoS One
Volume7
Issue number8
DOIs
Publication statusPublished - Aug 17 2012

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amniotic fluid
Cell proliferation
Amniotic Fluid
Stem cells
stroke
Grafts
stem cells
Rats
cell proliferation
Animals
Stem Cells
Stroke
Cell Proliferation
Transplants
Fluids
rats
animals
Lateral Ventricles
Middle Cerebral Artery Infarction
Dentate Gyrus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Intravenous grafts of amniotic fluid-derived stem cells induce endogenous cell proliferation and attenuate behavioral deficits in ischemic stroke rats. / Tajiri, Naoki; Acosta, Sandra; Glover, Loren E.; Bickford, Paula C.; Jacotte Simancas, Alejandra; Yasuhara, Takao; Date, Isao; Solomita, Marianna A.; Antonucci, Ivana; Stuppia, Liborio; Kaneko, Yuji; Borlongan, Cesar V.

In: PLoS One, Vol. 7, No. 8, e43779, 17.08.2012.

Research output: Contribution to journalArticle

Tajiri, N, Acosta, S, Glover, LE, Bickford, PC, Jacotte Simancas, A, Yasuhara, T, Date, I, Solomita, MA, Antonucci, I, Stuppia, L, Kaneko, Y & Borlongan, CV 2012, 'Intravenous grafts of amniotic fluid-derived stem cells induce endogenous cell proliferation and attenuate behavioral deficits in ischemic stroke rats', PLoS One, vol. 7, no. 8, e43779. https://doi.org/10.1371/journal.pone.0043779
Tajiri, Naoki ; Acosta, Sandra ; Glover, Loren E. ; Bickford, Paula C. ; Jacotte Simancas, Alejandra ; Yasuhara, Takao ; Date, Isao ; Solomita, Marianna A. ; Antonucci, Ivana ; Stuppia, Liborio ; Kaneko, Yuji ; Borlongan, Cesar V. / Intravenous grafts of amniotic fluid-derived stem cells induce endogenous cell proliferation and attenuate behavioral deficits in ischemic stroke rats. In: PLoS One. 2012 ; Vol. 7, No. 8.
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