Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice

Masafumi Shibamori, Keiki Ogino, Yasuhiro Kambayashi, Hironobu Ishiyama

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Airway responses induced by intranasal administration of mite allergen without adjuvant were studied in NC/Nga mice. A crude extract of Dermatophagoides farinae (Df) was administered for 5 consecutive days and a single intranasal challenge booster dose was given 1 week after the last sensitization. 24 h after the single challenge, the airway hyperresponsiveness (AHR) was measured and the bronchoalveolar lavage fluid (BALF) was analyzed for numbers of eosinophils and neutrophils, and both cytokine and chemokine levels. There were marked increases in number of eosinophils in the BALF, AHR, Th2 cytokines (IL-5 and IL-13), and chemokine (eotaxin-1 and eotaxin-2) levels in the BALF following Df exposure. C57BL/6N, A/J, BALB/c, and CBA/JN mouse strains were also exposed to Df crude extract, but all of the measured responses were strongest in NC/Nga mice. Furthermore, Df-exposed NC/Nga mice showed the goblet cell hyperplasia, pulmonary eosinophilic inflammation, and increases in both total serum IgE and Df-specific IgG1. After intranasal exposure of NC/Nga mice to crude extract of Dermatophagoides pteronyssinus, the BALF eosinophilia and AHR were similar to responses induced by Df. None of the study parameters were increased in response to intranasal exposure to ovalbumin. These data demonstrated that NC/Nga mice developed allergic asthma-like responses after intranasal exposure to mite allergens.

Original languageEnglish
Pages (from-to)987-994
Number of pages8
JournalLife Sciences
Volume78
Issue number9
DOIs
Publication statusPublished - Jan 25 2006

Keywords

  • Airway hyperresponsiveness
  • Airway inflammation
  • Intranasal administration
  • Mite allergen
  • NC/Nga mice

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Fingerprint Dive into the research topics of 'Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice'. Together they form a unique fingerprint.

  • Cite this