INTRALYMPHOCYTIC FREE CALCIUM AND MAGNESIUM IN STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RATS AND EFFECTS OF BLOOD PRESSURE AND VARIOUS ANTIHYPERTENSIVE AGENTS

Minoru Adachi, Yasuo Nara, Masayuki Mano, Katumi Ikeda, Ryoichi Horie, Yukio Yamori

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

1. Free Ca2+ ([Ca2+]i) and Mg2+ ([Mg2+]i) were measured in peripheral lymphocytes from stroke‐prone spontaneously hypertensive rats (SHRSP) and normotensive Wistar‐Kyoto rats (WKY) at the age of 5, 7 and 17 weeks, from various antihypertensive agents‐treated SHRSP, and from secondary hypertensive WKY. 2. At the age of 5 weeks, no difference was observed in systolic blood pressure (SBP), or lymphocyte [Ca2+]i and [Mg2+]i between SHRSP and WKY. At the age of 7 or 17 weeks, SBP and [Ca2+]i of SHRSP were significantly higher than in WKY, and at the age of 17 weeks, [Mg2+]i of SHRSP was significantly lower than in WKY. Further, [Ca2+]i or [Mg2+]i was positively or negatively correlated to SBP, and [Mg2+]i was negatively correlated to [Ca2+]i. 3. SBP of SHRSP fell significantly after antihypertensive treatment with calcium antagonist, angiotensin‐converting enzyme (ACE) inhibitor or hydralazine for 40 days. [Ca2+]i was significantly lower in calcium antagonist and hydralazine groups, and tended to be low in ACE inhibitor group. These four groups showed no difference in [Mg2+]i. 4. After 40‐day administration of NG‐nitro‐l‐arginine (l‐NNA), WKY developed severe hypertension, but there were no significant differences in lymphocyte [Ca2+]i and [Mg2+]i between the l‐NNA treated and non‐treated groups. 5. These results suggested that increased lymphocyte [Ca2+]i and decreased [Mg2+]i observed in SHRSP are not only secondary to hypertension but possibly related to a basic genetic abnormality of divalent cation handling.

Original languageEnglish
Pages (from-to)587-593
Number of pages7
JournalClinical and Experimental Pharmacology and Physiology
Volume20
Issue number9
DOIs
Publication statusPublished - Sep 1993

Keywords

  • N‐nitro‐l‐arginine
  • antihypertensive therapy
  • blood pressure
  • intralymphocytic free Ca
  • intralymphocytic free Mg
  • stroke‐prone spontaneously hypertensive rat.

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

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