Intragenic rearrangements of a mycoreovirus induced by the multifunctional protein p29 encoded by the prototypic hypovirus CHV1-EP713

Liying Sun, Nobuhiro Suzuki

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Mycoreovirus 1 (MyRV1), a member of the Reoviridae family possessing a genome consisting of 11 dsRNA segments (S1-S11), and the prototype hypovirus (CHV1-EP713) of the Hypoviridae family, which is closely related to the monopartite picorna-like superfamily with a ssRNA genome, infect the chestnut blight fungus and cause virulence attenuation and distinct phenotypic alterations in the host. Here, we present evidence for reproducible induction of intragenic rearrangements of MyRV1 S6 and S10, mediated by the multifunctional protein p29 encoded by CHV1. S6 and S10 underwent an almost full-length ORF duplication (S6L) and an internal deletion of three-fourths of the ORF (S10ss). No significant influence on symptom induction in the fungal host was associated with the S6L rearrangement. In contrast, S10-encoded VP10, while nonessential for MyRV1 replication, was shown to contribute to virulence reduction and reduced growth of aerial mycelia. Furthermore, p29 was found to copurify with MyRV1 genomic RNA and bind to VP9 in vitro and in vivo, suggesting direct interactions of p29 with the MyRV1 replication machinery. This study provides the first example of a viral factor involved in RNA genome rearrangements of a different virus and shows its usefulness as a probe into the mechanism of replication and symptom expression of a heterologous virus. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish
Pages (from-to)2557-2571
Number of pages15
JournalRNA
Volume14
Issue number12
DOIs
Publication statusPublished - Dec 2008

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Reoviridae
S 10
S 6
Proteins
Genome
Open Reading Frames
Virulence
RNA
Viruses
Mycelium
Fungi
Growth

Keywords

  • Hypovirus
  • Mycoreovirus
  • RNA rearrangement
  • RNA silencing suppressor

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Intragenic rearrangements of a mycoreovirus induced by the multifunctional protein p29 encoded by the prototypic hypovirus CHV1-EP713. / Sun, Liying; Suzuki, Nobuhiro.

In: RNA, Vol. 14, No. 12, 12.2008, p. 2557-2571.

Research output: Contribution to journalArticle

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