Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level

R. Kawano, Y. Tsuji, M. Hirano, T. Osaki, K. Kamiya, N. Miki, Toru Ide, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes an investigation of drug effects for ion channels with recognizing the intra/extracellular directions. We have previously reported the ion channel recordings with bilayer lipid membranes (BLMs) which formed on a parylene micropore using droplets contact method. However, the low reconstitution probability of ion channels into BLMs has been a major issue. In this study, BLMs area and position are regulated with changing the numbers and positions of the parylene micropores. As a result, the simultaneous single channel recordings of K+ channel expressed in nerve system with BLM array were able to be achieved at the high probability.

Original languageEnglish
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages57-58
Number of pages2
DOIs
Publication statusPublished - 2013
Externally publishedYes
EventIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
Duration: Jan 20 2013Jan 24 2013

Other

OtherIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
CountryTaiwan, Province of China
CityTaipei
Period1/20/131/24/13

Fingerprint

Lipid bilayers
Membrane Lipids
Ion Channels
lipids
membranes
Molecules
Ions
molecules
recording
nerves
Contacts (fluid mechanics)
drugs
Pharmaceutical Preparations
parylene

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Kawano, R., Tsuji, Y., Hirano, M., Osaki, T., Kamiya, K., Miki, N., ... Takeuchi, S. (2013). Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 57-58). [6474175] https://doi.org/10.1109/MEMSYS.2013.6474175

Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level. / Kawano, R.; Tsuji, Y.; Hirano, M.; Osaki, T.; Kamiya, K.; Miki, N.; Ide, Toru; Takeuchi, S.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. p. 57-58 6474175.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kawano, R, Tsuji, Y, Hirano, M, Osaki, T, Kamiya, K, Miki, N, Ide, T & Takeuchi, S 2013, Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 6474175, pp. 57-58, IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013, Taipei, Taiwan, Province of China, 1/20/13. https://doi.org/10.1109/MEMSYS.2013.6474175
Kawano R, Tsuji Y, Hirano M, Osaki T, Kamiya K, Miki N et al. Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. p. 57-58. 6474175 https://doi.org/10.1109/MEMSYS.2013.6474175
Kawano, R. ; Tsuji, Y. ; Hirano, M. ; Osaki, T. ; Kamiya, K. ; Miki, N. ; Ide, Toru ; Takeuchi, S. / Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. pp. 57-58
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