Intradermal injection of monocyte chemoattractant protein-1 induces emigration and differentiation of blood monocytes in rat skin

Shigeo Yamashiro, Motohiro Takeya, Jun Ichi Kuratsu, Yukitaka Ushio, Kiyoshi Takahashi, Teizo Yoshimura

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Monocyte chemoattractant protein-1 (MCP-1) is a potent chemoattractant for blood monocytes in vitro. Recent studies in MCP-1-transgenic mice revealed that the local production of MCP-1 caused monocyte infiltration. However, the kinetics of monocyte infiltration after the production of MCP-1 or the amount of MCP-1 necessary for monocyte recruitment are not known. Methods: We purified recombinant rat MCP-1 expressed in COS-7 cells, and injected it into rat skin. The infiltrating cells were examined by immunohistochemistry and ultrastructural peroxidase cytochemistry. Results: Rat recombinant MCP-1 had a molecular mass of approximately 30 kD and exhibited the peak monocyte chemotactic activity at 10-9 M. One microgram of MCP-1 caused intra- and extravascular accumulation of mononuclear cells 3 h after injection. The cells were ED1+, indicating they were blood monocytes. The infiltration of mononuclear cells peaked at 12-24 h: and most of them were TRPM-3+ and ED3+, characteristic to exudate macrophages. None of the cells expressed ED2 or Ki-M2R antigens, markers for resident macrophages, until 3 days after injection. There was no uptake of [3H]thymidine by the infiltrating cells. Ultrastructural peroxidase cytochemistry confirmed that the infiltrating cells were monocytes and exudate macrophages. The number of OX8+ lymphocytes also peaked at 12 h, consisting of approximately 9% of the total infiltrating cells. Conclusion: These results indicate that MCP-1 attracts blood monocytes as early as 3 h and the infiltrating monocytes differentiate into exudate macrophages in loco. However, this effect was transient and the infiltration of monocytes did not result in tissue damage.

Original languageEnglish
Pages (from-to)15-23
Number of pages9
JournalInternational archives of allergy and immunology
Volume115
Issue number1
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

Keywords

  • Chemokine
  • Chemotactic factor
  • Immunohistochemistry
  • Lymphocyte
  • Macrophage subpopulation
  • Monocyte chemoattractant protein-1
  • Ultrastructural study

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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