Intradermal injection of monocyte chemoattractant protein-1 induces emigration and differentiation of blood monocytes in rat skin

Shigeo Yamashiro, Motohiro Takeya, Jun Ichi Kuratsu, Yukitaka Ushio, Kiyoshi Takahashi, Teizo Yoshimura

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Monocyte chemoattractant protein-1 (MCP-1) is a potent chemoattractant for blood monocytes in vitro. Recent studies in MCP-1-transgenic mice revealed that the local production of MCP-1 caused monocyte infiltration. However, the kinetics of monocyte infiltration after the production of MCP-1 or the amount of MCP-1 necessary for monocyte recruitment are not known. Methods: We purified recombinant rat MCP-1 expressed in COS-7 cells, and injected it into rat skin. The infiltrating cells were examined by immunohistochemistry and ultrastructural peroxidase cytochemistry. Results: Rat recombinant MCP-1 had a molecular mass of approximately 30 kD and exhibited the peak monocyte chemotactic activity at 10-9 M. One microgram of MCP-1 caused intra- and extravascular accumulation of mononuclear cells 3 h after injection. The cells were ED1+, indicating they were blood monocytes. The infiltration of mononuclear cells peaked at 12-24 h: and most of them were TRPM-3+ and ED3+, characteristic to exudate macrophages. None of the cells expressed ED2 or Ki-M2R antigens, markers for resident macrophages, until 3 days after injection. There was no uptake of [3H]thymidine by the infiltrating cells. Ultrastructural peroxidase cytochemistry confirmed that the infiltrating cells were monocytes and exudate macrophages. The number of OX8+ lymphocytes also peaked at 12 h, consisting of approximately 9% of the total infiltrating cells. Conclusion: These results indicate that MCP-1 attracts blood monocytes as early as 3 h and the infiltrating monocytes differentiate into exudate macrophages in loco. However, this effect was transient and the infiltration of monocytes did not result in tissue damage.

Original languageEnglish
Pages (from-to)15-23
Number of pages9
JournalInternational Archives of Allergy and Immunology
Volume115
Issue number1
DOIs
Publication statusPublished - Jan 1998
Externally publishedYes

Fingerprint

Intradermal Injections
Chemokine CCL2
Emigration and Immigration
Monocytes
Skin
Exudates and Transudates
Macrophages
Histocytochemistry
Peroxidase
Injections
COS Cells
Chemotactic Factors
Lymphocyte Count
Recombinant Proteins
Thymidine
Transgenic Mice
Immunohistochemistry

Keywords

  • Chemokine
  • Chemotactic factor
  • Immunohistochemistry
  • Lymphocyte
  • Macrophage subpopulation
  • Monocyte chemoattractant protein-1
  • Ultrastructural study

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Intradermal injection of monocyte chemoattractant protein-1 induces emigration and differentiation of blood monocytes in rat skin. / Yamashiro, Shigeo; Takeya, Motohiro; Kuratsu, Jun Ichi; Ushio, Yukitaka; Takahashi, Kiyoshi; Yoshimura, Teizo.

In: International Archives of Allergy and Immunology, Vol. 115, No. 1, 01.1998, p. 15-23.

Research output: Contribution to journalArticle

Yamashiro, Shigeo ; Takeya, Motohiro ; Kuratsu, Jun Ichi ; Ushio, Yukitaka ; Takahashi, Kiyoshi ; Yoshimura, Teizo. / Intradermal injection of monocyte chemoattractant protein-1 induces emigration and differentiation of blood monocytes in rat skin. In: International Archives of Allergy and Immunology. 1998 ; Vol. 115, No. 1. pp. 15-23.
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