Intracellular trafficking of β2-glycoprotein I complexes with lipid vesicles in macrophages: Implications on the development of antiphospholipid syndrome

Toshimitsu Kajiwara, Tatsuji Yasuda, Eiji Matsuura

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

β2-Glycoprotein I (β2GPI) is known as a major autoantigen for antiphospholipid antibodies. Our recent data show that binding of β2GPI to oxidized low-density lipoprotein (oxLDL) or to liposomes containing anionic phospholipid(s) may facilitate the presentation of β2GPI's epitope by macrophages/dendritic cells to autoreactive T cells. In the present study, we investigated intracellular trafficking of β2GPI and its complexes with oxLDL or liposomes containing phosphatidylserine (PS-liposomes) in mouse macrophage-like J774 cells. A relatively small amount of non-complexed β2GPI was taken up and stagnated in the late endosome after incubating for 16 h. In contrast, β2GPI complexes with oxLDL or PS-liposomes were transported into the lysosome. In the presence of the IgG anti-β2GPI autoantibody, WB-CAL-1, β2GPI/oxLDL complexes were rapidly incorporated into intracellular space and were finally localized in the lysosome. Interestingly, in vitro pulses by β2GPI/oxLDL complexes together with WB-CAL-1 led to the expression of membranous CD36 as well as Fcγ type I receptors (FcγRI). These observations suggest that IgG immune complexes of β2GPI/oxLDL provide not only FcγRI- but also scavenger receptor-mediated uptake of β2GPI/oxLDL complexes by macrophages. Thus, β2GPI/oxLDL complexes as a major atherogenic autoantigen and IgG anti-β2GPI autoantibodies may facilitate antigen presentation and foam cell formation in antiphospholipid syndrome.

Original languageEnglish
Pages (from-to)164-173
Number of pages10
JournalJournal of Autoimmunity
Volume29
Issue number2-3
DOIs
Publication statusPublished - Sep 2007

Fingerprint

Antiphospholipid Syndrome
Glycoproteins
Macrophages
Lipids
Liposomes
Autoantigens
Lysosomes
Autoantibodies
Intracellular Space
Scavenger Receptors
Foam Cells
Antiphospholipid Antibodies
Fc Receptors
oxidized low density lipoprotein
Endosomes
Phosphatidylserines
Antigen Presentation
Antigen-Antibody Complex
Dendritic Cells
Epitopes

Keywords

  • β-Glycoprotein I
  • Antiphospholipid antibodies
  • Antiphospholipid syndrome
  • Intracellular trafficking
  • Oxidized low-density lipoprotein

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Intracellular trafficking of β2-glycoprotein I complexes with lipid vesicles in macrophages : Implications on the development of antiphospholipid syndrome. / Kajiwara, Toshimitsu; Yasuda, Tatsuji; Matsuura, Eiji.

In: Journal of Autoimmunity, Vol. 29, No. 2-3, 09.2007, p. 164-173.

Research output: Contribution to journalArticle

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