Intracellular pH and proton-transport in barley root cells under salt stress: In vivo 31P-NMR study

Maki Katsuhara, Yoshiaki Yazaki, Katsuhiro Sakano, Toshio Kawasaki

    Research output: Contribution to journalArticlepeer-review

    19 Citations (Scopus)

    Abstract

    Salt stress-induced changes of intracellular pH and in levels of phosphorous compounds were monitored in intact root tips of barley seedlings (Hordeum vulgare cv. Akashinriki) by in vivo 31P-nuclear magnetic resonance (NMR) spectroscopy. Vacuolar alkalization was observed after treatment with both 300 and 500 mM NaCl. Much of the observed apparent alkalization of the cytoplasm was eliminated when the effect of Na+ ions on the titration curve was considered. Within 1 h after the initiation of salt stress, levels of glucose-6-phosphate and UDP-glucose decreased markedly, and such decreases might lead directly or indirectly to cell death. Simultaneous measurements of the external and intracellular pH revealed the promotion of external acidification and internal alkalization during salt stress. Possible mechanisms of Na+/H+ antiport at the tonoplast and the role of proton-pump in the plasma membrane are discussed.

    Original languageEnglish
    Pages (from-to)155-160
    Number of pages6
    JournalPlant and Cell Physiology
    Volume38
    Issue number2
    DOIs
    Publication statusPublished - Feb 1997

    Keywords

    • Barley root
    • Cytoplasmic pH
    • In vivo P-NMR
    • Na/H antiport
    • Salt stress
    • Vacuolar pH

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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