Intracellular pH and proton-transport in barley root cells under salt stress: In vivo 31P-NMR study

Maki Katsuhara, Yoshiaki Yazaki, Katsuhiro Sakano, Toshio Kawasaki

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Salt stress-induced changes of intracellular pH and in levels of phosphorous compounds were monitored in intact root tips of barley seedlings (Hordeum vulgare cv. Akashinriki) by in vivo 31P-nuclear magnetic resonance (NMR) spectroscopy. Vacuolar alkalization was observed after treatment with both 300 and 500 mM NaCl. Much of the observed apparent alkalization of the cytoplasm was eliminated when the effect of Na+ ions on the titration curve was considered. Within 1 h after the initiation of salt stress, levels of glucose-6-phosphate and UDP-glucose decreased markedly, and such decreases might lead directly or indirectly to cell death. Simultaneous measurements of the external and intracellular pH revealed the promotion of external acidification and internal alkalization during salt stress. Possible mechanisms of Na+/H+ antiport at the tonoplast and the role of proton-pump in the plasma membrane are discussed.

Original languageEnglish
Pages (from-to)155-160
Number of pages6
JournalPlant and Cell Physiology
Volume38
Issue number2
DOIs
Publication statusPublished - Feb 1997

Keywords

  • Barley root
  • Cytoplasmic pH
  • In vivo P-NMR
  • Na/H antiport
  • Salt stress
  • Vacuolar pH

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Intracellular pH and proton-transport in barley root cells under salt stress: In vivo <sup>31</sup>P-NMR study'. Together they form a unique fingerprint.

  • Cite this