Intracellular interaction of EMILIN-1 with fibrillin-1 in human periodontal ligament cells

Yuka Nakatomi, Eichi Tsuruga, Yoshinori Yamauchi, Megumi Kawagoe, Kaori Yamanouchi, Kazuki Nakashima, Yoshihiko Sawa, Hiroyuki Ishikawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The elastic system fibers comprise oxytalan, elaunin and elastic fibers, differing in their relative microfibril and elastin contents. Human periodontal ligaments (PDLs) contain oxytalan fibers (pure microfibrils), which are composed mainly of fibrillin-1, the major component of microfibrils. We recently demonstrated that EMILIN-1, located at the interface between elastin and microfibrils, controls the amount of fibrillin-1 assembly in PDL fibroblast cell/matrix layers [8], although the mechanism involved was unclear. We subjected cultured PDL fibroblasts to immunofluorescence and immunoprecipitation assays in order to examine the intracellular interaction of EMILIN-1 with fibrillin-1. Immunofluorescence showed that EMILIN-1 was colocalized with fibrillin-1, both inside and outside the cells. Additionally, EMILIN-1 formed a complex with fibrillin-1 in the intracellular fraction. These results suggest that EMILIN-1 may form complexes with fibrillin-1 in cellular vesicles, thus contributing effectively to the initial assembly of pericellular fibrillin-1 during the process of oxytalan fiber formation.

Original languageEnglish
Pages (from-to)66-69
Number of pages4
JournalOrthodontic Waves
Volume71
Issue number2
DOIs
Publication statusPublished - Jun 1 2012
Externally publishedYes

Keywords

  • EMILIN
  • Fibrillin
  • Microfibrils
  • Oxytalan fiber
  • Periodontal ligament

ASJC Scopus subject areas

  • Orthodontics

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  • Cite this

    Nakatomi, Y., Tsuruga, E., Yamauchi, Y., Kawagoe, M., Yamanouchi, K., Nakashima, K., Sawa, Y., & Ishikawa, H. (2012). Intracellular interaction of EMILIN-1 with fibrillin-1 in human periodontal ligament cells. Orthodontic Waves, 71(2), 66-69. https://doi.org/10.1016/j.odw.2012.01.002