Intracellular distribution of desmoplakin in human odontoblasts

Yoshihiko Sawa, Shin Ichiro Kuroshima, Yuji Yamaoka, Shigemitsu Yoshida

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Coexpression of desmosomal proteins and vimentin has been reported in a specific mesenchymal phenotype. This study investigated the expression of vimentin-binding desmosomal proteins in human dental pulp fibroblasts (DPF) and odontoblasts. The dental pulp has no cells expressing desmocollin (DSC) 1-3, desmoglein (DSG) 1-3, junction plakoglobin (JUP), or desmoplakin (DPK) 1 and 2 except for odontoblasts expressing DPK. A confocal image by laser-scanning microscopy demonstrated the diffuse distribution of DPK in the cytoplasm throughout the odontoblast processes. In culture, the mRNA expression of JUP and DPK1, but not DSC1-3 and DSG1-3, was detected in all DPF clones tested and also in odontoblast-like cells (OB) expressing osteocalcin and dentin sialophosphoprotein mRNAs established in the differentiation medium. The DPF having the potential to differentiate into OB expressed vimentin, but not DPK before culturing in the differentiation medium, whereas OB expressed vimentin-binding DPK1. These results suggest that DPF usually expresses DPK1 mRNA, and that the DPK1 production and the bonding of vimentin to DPK1 occur in DPF with the differentiation into odontoblasts.

Original languageEnglish
Pages (from-to)1099-1108
Number of pages10
JournalJournal of Histochemistry and Cytochemistry
Volume53
Issue number9
DOIs
Publication statusPublished - Sep 2005
Externally publishedYes

Fingerprint

Desmoplakins
Odontoblasts
Dental Pulp
Vimentin
Fibroblasts
gamma Catenin
Messenger RNA
Desmocollins
Desmoglein 3
Desmoglein 1
Osteocalcin
Confocal Microscopy
Carrier Proteins
Cytoplasm
Clone Cells
Phenotype

Keywords

  • Desmoplakin
  • Odontoblasts
  • Vimentin

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Intracellular distribution of desmoplakin in human odontoblasts. / Sawa, Yoshihiko; Kuroshima, Shin Ichiro; Yamaoka, Yuji; Yoshida, Shigemitsu.

In: Journal of Histochemistry and Cytochemistry, Vol. 53, No. 9, 09.2005, p. 1099-1108.

Research output: Contribution to journalArticle

Sawa, Yoshihiko ; Kuroshima, Shin Ichiro ; Yamaoka, Yuji ; Yoshida, Shigemitsu. / Intracellular distribution of desmoplakin in human odontoblasts. In: Journal of Histochemistry and Cytochemistry. 2005 ; Vol. 53, No. 9. pp. 1099-1108.
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