TY - JOUR
T1 - Intra-arterial injection of prolactin-releasing peptide elevates prolactin gene expression and plasma prolactin levels in rainbow trout
AU - Sakamoto, T.
AU - Agustsson, T.
AU - Moriyama, S.
AU - Itoh, T.
AU - Takahashi, A.
AU - Kawauchi, H.
AU - Björnsson, B. Th
AU - Ando, M.
N1 - Funding Information:
Acknowledgements We thank Prof. Masao Ono for providing the cDNA probe encoding chum salmon somatolactin. This work was supported by grant-in-aid for scientific research from the Ministry of Education, the Fisheries Agency of Japan and the Swedish Council for Forestry and Agricultural Research. The experimental work was approved by the Ethical Committee on Animal Research in Göteborg and subsequently carried out under license 169.98 in compliance with the standards and procedures laid down by the Swedish Ministry of Agriculture.
PY - 2003/6
Y1 - 2003/6
N2 - Prolactin-releasing peptide (PrRP), recently isolated from the brain of mammals and teleosts, is a strong candidate for being a stimulatory hormone of pituitary prolactin secretion. The present study examined whether or not PrRP is capable of inducing prolactin gene expression and elevating plasma prolactin levels in vivo in cannulated rainbow trout. Following a single intra-arterial injection of chum salmon PrRP (40 nmol kg-1) through a dorsal aorta catheter, plasma prolactin levels increased (P < 0.05) rapidly (2 min and 30 min), and prolactin mRNA levels were elevated (P < 0.05) in pituitaries sampled 8 h after the injection. In contrast, plasma levels of somatolactin were decreased (P < 0.05) and growth hormone and somatolactin mRNA levels were not significantly affected by PrRP. Thus, PrRP appears to be a potent prolactin secretagogue as well as prolactin transcription inducer in vivo in the rainbow trout.
AB - Prolactin-releasing peptide (PrRP), recently isolated from the brain of mammals and teleosts, is a strong candidate for being a stimulatory hormone of pituitary prolactin secretion. The present study examined whether or not PrRP is capable of inducing prolactin gene expression and elevating plasma prolactin levels in vivo in cannulated rainbow trout. Following a single intra-arterial injection of chum salmon PrRP (40 nmol kg-1) through a dorsal aorta catheter, plasma prolactin levels increased (P < 0.05) rapidly (2 min and 30 min), and prolactin mRNA levels were elevated (P < 0.05) in pituitaries sampled 8 h after the injection. In contrast, plasma levels of somatolactin were decreased (P < 0.05) and growth hormone and somatolactin mRNA levels were not significantly affected by PrRP. Thus, PrRP appears to be a potent prolactin secretagogue as well as prolactin transcription inducer in vivo in the rainbow trout.
KW - Growth hormone
KW - Pituitary
KW - Prolactin
KW - Prolactin-releasing peptide
KW - Somatolactin
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U2 - 10.1007/s00360-003-0340-1
DO - 10.1007/s00360-003-0340-1
M3 - Article
C2 - 12687398
AN - SCOPUS:0038270072
SN - 0174-1578
VL - 173
SP - 333
EP - 337
JO - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
JF - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
IS - 4
ER -