Intra-arterial injection of prolactin-releasing peptide elevates prolactin gene expression and plasma prolactin levels in rainbow trout

Tatsuya Sakamoto, T. Agustsson, S. Moriyama, T. Itoh, A. Takahashi, H. Kawauchi, B. Th Björnsson, M. Ando

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Prolactin-releasing peptide (PrRP), recently isolated from the brain of mammals and teleosts, is a strong candidate for being a stimulatory hormone of pituitary prolactin secretion. The present study examined whether or not PrRP is capable of inducing prolactin gene expression and elevating plasma prolactin levels in vivo in cannulated rainbow trout. Following a single intra-arterial injection of chum salmon PrRP (40 nmol kg-1) through a dorsal aorta catheter, plasma prolactin levels increased (P <0.05) rapidly (2 min and 30 min), and prolactin mRNA levels were elevated (P <0.05) in pituitaries sampled 8 h after the injection. In contrast, plasma levels of somatolactin were decreased (P <0.05) and growth hormone and somatolactin mRNA levels were not significantly affected by PrRP. Thus, PrRP appears to be a potent prolactin secretagogue as well as prolactin transcription inducer in vivo in the rainbow trout.

Original languageEnglish
Pages (from-to)333-337
Number of pages5
JournalJournal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
Volume173
Issue number4
DOIs
Publication statusPublished - Jun 2003

Fingerprint

Prolactin-Releasing Hormone
Intra-Arterial Injections
Oncorhynchus mykiss
prolactin
Gene expression
Prolactin
peptide
rainbow
gene expression
peptides
injection
Plasmas
Gene Expression
plasma
hormone
Oncorhynchus keta
teleost
secretion
Messenger RNA
brain

Keywords

  • Growth hormone
  • Pituitary
  • Prolactin
  • Prolactin-releasing peptide
  • Somatolactin

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Environmental Science(all)
  • Physiology
  • Physiology (medical)

Cite this

Intra-arterial injection of prolactin-releasing peptide elevates prolactin gene expression and plasma prolactin levels in rainbow trout. / Sakamoto, Tatsuya; Agustsson, T.; Moriyama, S.; Itoh, T.; Takahashi, A.; Kawauchi, H.; Björnsson, B. Th; Ando, M.

In: Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, Vol. 173, No. 4, 06.2003, p. 333-337.

Research output: Contribution to journalArticle

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AU - Itoh, T.

AU - Takahashi, A.

AU - Kawauchi, H.

AU - Björnsson, B. Th

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