Intra- and intermolecular interactions of the catalytic domains of human CD45 protein tyrosine phosphatase

Keiko Hayami-Noumi, Tomofusa Tsuchiya, Yoshinori Moriyama, Takato Noumi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have investigated protein-protein interaction between distinct domains of the human CD45 cytoplasmic region using a yeast two-hybrid system. Consequently, we have found that the spacer region between two tandem PTP domains specifically interacts with the membrane-distal PTP domain (D2). This interaction is mediated by a stretch of amino acid residues in the carboxyl-terminal half of the spacer region. Although the membrane proximal region does not directly interact with either of the two PTP domains, it appears to function in stabilizing the interaction between the spacer region and D2. We also demonstrate that the interaction between the spacer region and D2 might take place intramolecularly. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)68-72
Number of pages5
JournalFEBS Letters
Volume468
Issue number1
DOIs
Publication statusPublished - Feb 18 2000

Keywords

  • CD45
  • Protein tyrosine phosphatase
  • Protein-protein interaction
  • Two-hybrid system

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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