Abstract
The intestinal expression of the CYP3A subfamily was investigated in female rats, and the intestinal metabolism of two CYP3A substrates, testosterone and rifabutin, was examined and compared between males and females. CYP3A1/23 and CYP3A2 intestinal expression was barely detected in male and female rats. Although CYP3A9 was predominantly expressed in the female rat liver, its expression in the intestine was not different between the two sexes. The rate of testosterone 6β-hydroxylation in the female intestine was similar to that for males. Rifabutin was also metabolized at similar rates in both intestines, although the metabolic rate was greater in the female liver. These results indicate that the intestinal drug metabolizing activity of the CYP3A subfamily is similar between males and females, and that CYP3A9 is involved in the intestinal metabolism of CYP3A substrates in both sexes.
| Original language | English |
|---|---|
| Pages (from-to) | 311-315 |
| Number of pages | 5 |
| Journal | Biological and Pharmaceutical Bulletin |
| Volume | 28 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2005 |
| Externally published | Yes |
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Keywords
- CYP3A9
- Intestinal metabolism
- Rifabutin
- Testosterone
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
Intestinal expression and metabolic activity of the CYP3A subfamily in female rats. / Aiba, Tetsuya; Yoshinaga, Manko; Ishida, Kazuya; Takehara, Yutaka; Hashimoto, Yukiya.
In: Biological and Pharmaceutical Bulletin, Vol. 28, No. 2, 02.2005, p. 311-315.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intestinal expression and metabolic activity of the CYP3A subfamily in female rats
AU - Aiba, Tetsuya
AU - Yoshinaga, Manko
AU - Ishida, Kazuya
AU - Takehara, Yutaka
AU - Hashimoto, Yukiya
PY - 2005/2
Y1 - 2005/2
N2 - The intestinal expression of the CYP3A subfamily was investigated in female rats, and the intestinal metabolism of two CYP3A substrates, testosterone and rifabutin, was examined and compared between males and females. CYP3A1/23 and CYP3A2 intestinal expression was barely detected in male and female rats. Although CYP3A9 was predominantly expressed in the female rat liver, its expression in the intestine was not different between the two sexes. The rate of testosterone 6β-hydroxylation in the female intestine was similar to that for males. Rifabutin was also metabolized at similar rates in both intestines, although the metabolic rate was greater in the female liver. These results indicate that the intestinal drug metabolizing activity of the CYP3A subfamily is similar between males and females, and that CYP3A9 is involved in the intestinal metabolism of CYP3A substrates in both sexes.
AB - The intestinal expression of the CYP3A subfamily was investigated in female rats, and the intestinal metabolism of two CYP3A substrates, testosterone and rifabutin, was examined and compared between males and females. CYP3A1/23 and CYP3A2 intestinal expression was barely detected in male and female rats. Although CYP3A9 was predominantly expressed in the female rat liver, its expression in the intestine was not different between the two sexes. The rate of testosterone 6β-hydroxylation in the female intestine was similar to that for males. Rifabutin was also metabolized at similar rates in both intestines, although the metabolic rate was greater in the female liver. These results indicate that the intestinal drug metabolizing activity of the CYP3A subfamily is similar between males and females, and that CYP3A9 is involved in the intestinal metabolism of CYP3A substrates in both sexes.
KW - CYP3A9
KW - Intestinal metabolism
KW - Rifabutin
KW - Testosterone
UR - http://www.scopus.com/inward/record.url?scp=19444375003&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19444375003&partnerID=8YFLogxK
U2 - 10.1248/bpb.28.311
DO - 10.1248/bpb.28.311
M3 - Article
C2 - 15684490
AN - SCOPUS:19444375003
VL - 28
SP - 311
EP - 315
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
SN - 0918-6158
IS - 2
ER -