Intestinal epithelial cells promote secretion of leptin and adiponectin in adipocytes

Rino Ishihara, Yuki Mizuno, Akiho Miwa, Akihiro Hamada, Takeshi Tsuruta, Martin Wabitsch, Kei Sonoyama

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Although leptin and adiponectin are the predominant adipokines, how their circulating levels are regulated is incompletely understood. The present study tested whether intestinal epithelial cells influence the expression and secretion of these adipokines by adipocytes. Leptin gene expression and secretion by cultured human primary adipocytes and Simpson-Golabi-Behmel Syndrome adipocytes increased upon coculture with human enterocytic Caco-2 cells or incubation in conditioned medium of Caco-2 cells. Although adiponectin secretion increased, its mRNA levels decreased. Tissue homogenate of the ileum (but not the jejunum, colon, or liver) of nonobese C57BL/6J mice also stimulated leptin and adiponectin secretion by cultured murine 3T3-L1 adipocytes. However, ileal homogenate of obese KK-Ay mice had no effect on leptin and adiponectin secretion. We propose that as yet unidentified humoral factors released from intestinal epithelial cells are involved in regulating circulating leptin and adiponectin levels. Decreased production of such factors may contribute to hyperphagia in KK-Ay mice.

Original languageEnglish
Pages (from-to)362-368
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - Mar 2 2015
Externally publishedYes


  • Adipocyte
  • Adiponectin
  • Intestine
  • Leptin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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