Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres

Masateru Nagae, Takumi Ikeda, Yasuo Mikami, Hitoshi Hase, Hitoshi Ozawa, Ken Ichi Matsuda, Hirotaka Sakamoto, Yasuhiko Tabata, Mitsuhiro Kawata, Toshikazu Kubo

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

This study evaluated the regenerative effects of platelet-rich plasma (PRP) for the degenerated intervertebral disc (IVD) in vivo. After induction of IVD degeneration in rabbits, we prepared PRP by centrifuging blood obtained from these rabbits. These PRP were injected into the nucleus pulposus (NP) of the degenerated IVDs after impregnation into gelatin hydrogel microspheres that can immobilize PRP growth factors physiochemically and release them in a sustained manner with the degradation of the microspheres. As controls, microspheres impregnated with phosphate-buffered saline (PBS) and PRP without microspheres were similarly injected. Histologically, notable progress in IVD degeneration with time courses was observed in the PBS control, PRP-only, and sham groups. In contrast, progress was remarkably suppressed over the 8-week period in the PRP group. Moreover, in immunohistochemistry, intense immunostaining for proteoglycan in the NP and inner layer of the annulus fibrosus was observed 8 weeks after administration of PRP-impregnated microspheres. Almost all microspheres were indistinct 8 weeks after the injection, and there were no apparent side effects in this study. Our results suggest that the combined administration of PRP and gelatin hydrogel microspheres into the IVD may be a promising therapeutic modality for IVD degeneration.

Original languageEnglish
Pages (from-to)147-158
Number of pages12
JournalTissue Engineering
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 2007
Externally publishedYes

Fingerprint

Platelet-Rich Plasma
Intervertebral Disc
Hydrogel
Gelatin
Platelets
Microspheres
Hydrogels
Regeneration
Plasmas
Intervertebral Disc Degeneration
Phosphates
Rabbits
Proteoglycans
Impregnation
Intercellular Signaling Peptides and Proteins
Blood
Immunohistochemistry
Degradation
Injections

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology

Cite this

Nagae, M., Ikeda, T., Mikami, Y., Hase, H., Ozawa, H., Matsuda, K. I., ... Kubo, T. (2007). Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres. Tissue Engineering, 13(1), 147-158. https://doi.org/10.1089/ten.2006.0042

Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres. / Nagae, Masateru; Ikeda, Takumi; Mikami, Yasuo; Hase, Hitoshi; Ozawa, Hitoshi; Matsuda, Ken Ichi; Sakamoto, Hirotaka; Tabata, Yasuhiko; Kawata, Mitsuhiro; Kubo, Toshikazu.

In: Tissue Engineering, Vol. 13, No. 1, 01.2007, p. 147-158.

Research output: Contribution to journalArticle

Nagae, M, Ikeda, T, Mikami, Y, Hase, H, Ozawa, H, Matsuda, KI, Sakamoto, H, Tabata, Y, Kawata, M & Kubo, T 2007, 'Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres', Tissue Engineering, vol. 13, no. 1, pp. 147-158. https://doi.org/10.1089/ten.2006.0042
Nagae, Masateru ; Ikeda, Takumi ; Mikami, Yasuo ; Hase, Hitoshi ; Ozawa, Hitoshi ; Matsuda, Ken Ichi ; Sakamoto, Hirotaka ; Tabata, Yasuhiko ; Kawata, Mitsuhiro ; Kubo, Toshikazu. / Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres. In: Tissue Engineering. 2007 ; Vol. 13, No. 1. pp. 147-158.
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