Internal detoxification mechanism of Al in hydrangea: Identification of Al form in the leaves

Jian Feng Ma, Syuntaro Hiradate, Kyosuke Nomoto, Takasi Iwashita, Hideaki Matsumoto

Research output: Contribution to journalArticle

210 Citations (Scopus)

Abstract

An internal detoxification mechanism for Al was investigated in an Al-accumulating plant, hydrangea (Hydrangea macrophylla), focusing on Al forms present in the cells. The leaves of hydrangea contained as much as 15.7 mmol Al kg-1 fresh weight, and more than two-thirds of the Al was found in the cell sap. Using 27Al-nuclear magnetic resonance, the dominant peak of Al was observed at a chemical shift of 11 to 12 parts per million in both intact leaves and the extracted cell sap, which is in good accordance with the chemical shift for the 1:1 Al-citrate complex. Purification of cell sap by molecular sieve chromatography (Sephadex G-10) combined with ion-exclusion chromatography indicated that Al in fractions with the same retention time as citric acid contributed to the observed 27Al peak in the intact leaves. The molar ratio of Al to citric acid in the crude and purified cell sap approximated 1. The structure of the ligand chelated with Al was identified to be citric acid. Bioassay experiments showed that the purified Al complex from the cell sap did not inhibit root elongation of corn (Zea mays L) and the viability of cells on the root tip surface was also not affected. These observations indicate that Al is bound to citric acid in the cells of hydrangea leaves.

Original languageEnglish
Pages (from-to)1033-1039
Number of pages7
JournalPlant Physiology
Volume113
Issue number4
Publication statusPublished - Apr 1997

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Hydrangea
Citric Acid
sap
citric acid
leaves
cells
Zea mays
Gel Chromatography
Hydrangea macrophylla
Meristem
ion exchange chromatography
root tips
citrates
Biological Assay
cell viability
nuclear magnetic resonance spectroscopy
root growth
Cell Survival
Magnetic Resonance Spectroscopy
bioassays

ASJC Scopus subject areas

  • Plant Science

Cite this

Ma, J. F., Hiradate, S., Nomoto, K., Iwashita, T., & Matsumoto, H. (1997). Internal detoxification mechanism of Al in hydrangea: Identification of Al form in the leaves. Plant Physiology, 113(4), 1033-1039.

Internal detoxification mechanism of Al in hydrangea : Identification of Al form in the leaves. / Ma, Jian Feng; Hiradate, Syuntaro; Nomoto, Kyosuke; Iwashita, Takasi; Matsumoto, Hideaki.

In: Plant Physiology, Vol. 113, No. 4, 04.1997, p. 1033-1039.

Research output: Contribution to journalArticle

Ma, JF, Hiradate, S, Nomoto, K, Iwashita, T & Matsumoto, H 1997, 'Internal detoxification mechanism of Al in hydrangea: Identification of Al form in the leaves', Plant Physiology, vol. 113, no. 4, pp. 1033-1039.
Ma JF, Hiradate S, Nomoto K, Iwashita T, Matsumoto H. Internal detoxification mechanism of Al in hydrangea: Identification of Al form in the leaves. Plant Physiology. 1997 Apr;113(4):1033-1039.
Ma, Jian Feng ; Hiradate, Syuntaro ; Nomoto, Kyosuke ; Iwashita, Takasi ; Matsumoto, Hideaki. / Internal detoxification mechanism of Al in hydrangea : Identification of Al form in the leaves. In: Plant Physiology. 1997 ; Vol. 113, No. 4. pp. 1033-1039.
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