Interleukins affect equine endometrial cell function: Modulatory action of ovarian steroids

Anna Z. Szóstek, Antonio M. Galvão, Takuo Hojo, Kiyoshi Okuda, Dariusz J. Skarzynski

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Abstract

The aim of the present study was to investigate the interaction between ovarian steroids, interleukins and prostaglandins (PG) in equine epithelial and stromal cells in vitro. In Experiment 1, cells were exposed to IL-1α (10 ng/mL), IL-1β (10 ng/mL) or IL-6 (10 ng/mL) for 24 h and cell proliferation was determined using MTT. In Experiment 2, cells were exposed to progesterone (P4; 10-7 M); 17-β estradiol (E2; 10 -9 M) or P4+E2 for 24 h and later medium was replaced with a fresh one treated with IL-1α, IL-1β or IL-6 (10 ng/mL, each) for 24 h. The oxytocin (OT; 10-7 M) was used as a positive control. In Experiment 3, cells were exposed to P4 (10 -7 M), E2 (10-9 M) or P4+E 2 for 24 h and the IL receptor mRNAs transcription was determined using Real-time PCR. Prostaglandins concentration was determined using the direct enzyme immunoassay (EIA) method. Our findings reveal a functional linking between ovarian steroids and IL-stimulated PG secretion by equine endometrial cells. This interaction could be one of the mechanisms responsible for endometrial local orchestrating events during the estrous cycle and early pregnancy.

Original languageEnglish
Article number208103
JournalMediators of Inflammation
Volume2014
DOIs
Publication statusPublished - 2014

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ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Medicine(all)

Cite this

Szóstek, A. Z., Galvão, A. M., Hojo, T., Okuda, K., & Skarzynski, D. J. (2014). Interleukins affect equine endometrial cell function: Modulatory action of ovarian steroids. Mediators of Inflammation, 2014, [208103]. https://doi.org/10.1155/2014/208103