Interleukin (IL)-8 and Growth Related Oncogene-α in Severe Endotoxemia and the Effects of a Tumor Necrosis Factor-α/IL-1β Inhibitor on These Chemokines

Pablo Rodríguez-Wilhelmi, Ramón Montes, Akihiro Matsukawa, Verónica Hurtado, Marta Montes, José Hermida, Eduardo Rocha

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

FR167653 inhibits the production of tumor necrosis factor (TNF)-α and interleukin (IL)-1β, powerful inducers of CXC chemokines IL-8 and growth related oncogene (GRO)-α. The production of IL-8 and GRO-α was investigated and the effects of FR167653 were examined in a rabbit model of endotoxin shock. Male New Zealand rabbits were given endotoxin at a dose sufficient to induce DIC. Three groups of rabbits received FR167653 at different doses. TNF-α, IL-1β, IL-8, and GRO-α levels were measured, several pathologic features were evaluated, and the results were compared with those obtained in control rabbits, which received only endotoxin. Endotoxin increased serum levels of IL-8 and GRO-α, which were associated with hypotension, renal dysfunction, and mortality, peaking at 4 h. FR167653 improved mortality, an event that was associated with decreased levels of not only TNF-α and IL-1β but also IL-8 and GRO-α. TNF-α peaked at 2 h, at a time point before IL-8 and GRO-α reached their peak, and the TNF-α level was tightly correlated with that of IL-8 and GRO-α. Altogether, these data suggest the possible involvement of IL-8 and GRO-α in endotoxin shock, and FR167653 may foster a beneficial outcome in part by modulating the chemokines level by inhibiting TNF-α and IL-1β.

Original languageEnglish
Pages (from-to)220-229
Number of pages10
JournalExperimental and Molecular Pathology
Volume73
Issue number3
DOIs
Publication statusPublished - Dec 2002
Externally publishedYes

Fingerprint

Endotoxemia
Interleukin-8
Interleukin-1
Oncogenes
Chemokines
Tumor Necrosis Factor-alpha
Endotoxins
Growth
Rabbits
Shock
CXC Chemokines
Dacarbazine
Mortality
Hypotension
FR 167653
Kidney
Serum

Keywords

  • Endotoxin shock
  • FR167653
  • Growth related oncogene-α
  • Interleukin-1β
  • Interleukin-8
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine

Cite this

Interleukin (IL)-8 and Growth Related Oncogene-α in Severe Endotoxemia and the Effects of a Tumor Necrosis Factor-α/IL-1β Inhibitor on These Chemokines. / Rodríguez-Wilhelmi, Pablo; Montes, Ramón; Matsukawa, Akihiro; Hurtado, Verónica; Montes, Marta; Hermida, José; Rocha, Eduardo.

In: Experimental and Molecular Pathology, Vol. 73, No. 3, 12.2002, p. 220-229.

Research output: Contribution to journalArticle

Rodríguez-Wilhelmi, Pablo ; Montes, Ramón ; Matsukawa, Akihiro ; Hurtado, Verónica ; Montes, Marta ; Hermida, José ; Rocha, Eduardo. / Interleukin (IL)-8 and Growth Related Oncogene-α in Severe Endotoxemia and the Effects of a Tumor Necrosis Factor-α/IL-1β Inhibitor on These Chemokines. In: Experimental and Molecular Pathology. 2002 ; Vol. 73, No. 3. pp. 220-229.
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