Interleukin-8 as an essential factor in the human chorionic gonadotropin-induced rabbit ovulatory process: Interleukin-8 induces neutrophil accumulation and activation in ovulation

Takeshi Ujioka, Akihiro Matsukawa, Nobuyuki Tanaka, Kohei Matsuura, Masaru Yoshinaga, Hitoshi Okamura

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The objective of our study was to elucidate the involvement of interleukin (IL)-8 in the hCG-induced rabbit ovulatory process. After administering hCG (100 IU i.v.), we examined myeloperoxidase (MPO) activity, which represents neutrophil accumulation; neutrophil elastase (NE) activity, which is an indicator of neutrophil activity; and levels of IL-8 in the ovaries. The maximal level of IL-8 was observed before MPO and NE activities reached a peak: production of IL-8, MPO, and NE activities peaked, respectively, at 4 h (5.58 ± 0.88 pg/mg ovary, n = 13), 6 h (1.07 ± 0.13 ΔA/min per gram ovary, n = 8), and 9 h (18.89 ± 1.05 U/g ovary, n = 8). Anti-rabbit IL-8 antiserum given i.v. significantly reduced the maximal levels of hCG-induced MPO activity (antiserum vs. control; 0.34 ± 0.04 vs. 1.12 ± 0.11 ΔA/min per gram ovary, n = 14, p <0.001) and NE activity (8.14 ± 0.85 vs. 18.30 ± 0.79 U/g ovary, n = 14, p <0.001). The hCG-induced ovulation rate was significantly inhibited by the antiserum (50.5% vs. 83.9%, n = 14, p <0.001). Intraperitoneal injection of 100 mg/kg of ONO-5046, a specific NE inhibitor, also attenuated the ovulation rate (ONO-5046 vs. vehicle; 56.0% vs. 74.0%, n = 14, p <0.05). These findings clearly indicate that IL-8 has an important role in the hCG-induced ovulatory process through the accumulation and activation of neutrophils.

Original languageEnglish
Pages (from-to)526-530
Number of pages5
JournalBiology of Reproduction
Volume58
Issue number2
DOIs
Publication statusPublished - Feb 1998
Externally publishedYes

Fingerprint

Neutrophil Activation
Chorionic Gonadotropin
Ovulation
Interleukin-8
Ovary
Leukocyte Elastase
Rabbits
Peroxidase
Immune Sera
Neutrophils
Secretory Proteinase Inhibitory Proteins
Intraperitoneal Injections

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Interleukin-8 as an essential factor in the human chorionic gonadotropin-induced rabbit ovulatory process : Interleukin-8 induces neutrophil accumulation and activation in ovulation. / Ujioka, Takeshi; Matsukawa, Akihiro; Tanaka, Nobuyuki; Matsuura, Kohei; Yoshinaga, Masaru; Okamura, Hitoshi.

In: Biology of Reproduction, Vol. 58, No. 2, 02.1998, p. 526-530.

Research output: Contribution to journalArticle

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abstract = "The objective of our study was to elucidate the involvement of interleukin (IL)-8 in the hCG-induced rabbit ovulatory process. After administering hCG (100 IU i.v.), we examined myeloperoxidase (MPO) activity, which represents neutrophil accumulation; neutrophil elastase (NE) activity, which is an indicator of neutrophil activity; and levels of IL-8 in the ovaries. The maximal level of IL-8 was observed before MPO and NE activities reached a peak: production of IL-8, MPO, and NE activities peaked, respectively, at 4 h (5.58 ± 0.88 pg/mg ovary, n = 13), 6 h (1.07 ± 0.13 ΔA/min per gram ovary, n = 8), and 9 h (18.89 ± 1.05 U/g ovary, n = 8). Anti-rabbit IL-8 antiserum given i.v. significantly reduced the maximal levels of hCG-induced MPO activity (antiserum vs. control; 0.34 ± 0.04 vs. 1.12 ± 0.11 ΔA/min per gram ovary, n = 14, p <0.001) and NE activity (8.14 ± 0.85 vs. 18.30 ± 0.79 U/g ovary, n = 14, p <0.001). The hCG-induced ovulation rate was significantly inhibited by the antiserum (50.5{\%} vs. 83.9{\%}, n = 14, p <0.001). Intraperitoneal injection of 100 mg/kg of ONO-5046, a specific NE inhibitor, also attenuated the ovulation rate (ONO-5046 vs. vehicle; 56.0{\%} vs. 74.0{\%}, n = 14, p <0.05). These findings clearly indicate that IL-8 has an important role in the hCG-induced ovulatory process through the accumulation and activation of neutrophils.",
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