Abstract
Leukocyte accumulation and activation are key events in the pathogenesis of inflammatory lung disease. The ability of human airway smooth muscle cells (HASM) to contribute to the inflammatory process by its ability to produce the chemokines interleukin (IL) 8, monocyte chemotactic protein (MCP-1) and regulated on activation, normal T cell expressed and secreted (RANTES) was investigated. Cultured HASM, when stimulated with the pro-inflammatory cytokines IL-1α (0.01-2 ng/ml) or tumour necrosis factor or (TNF-α, 0.3-30 ng/ml), synthesize and release substantial amounts of IL-8, as assessed by specific immunoassay, bioasssay (elevation of intracellular free calcium in human neutrophils), and upregulation of mRNA. These stimuli also increased MCP-1 production and mRNA expression, but RANTES mRNA expression was not detected at 24 h. The smooth muscle spasmogen endothelin 1 (1 μM) was unable to stimulate IL-8 or MCP-1 release or mRNA expression. These data indicate that HASM may constitute an important source of leukocyte attractants in the inflamed lung, where the inducing stimuli, IL-1α and TNF-α, are also likely to be present.
| Original language | English |
|---|---|
| Pages (from-to) | 346-352 |
| Number of pages | 7 |
| Journal | Cytokine |
| Volume | 10 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 1998 |
| Externally published | Yes |
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Keywords
- Airway smooth muscle
- Chemokines
- IL-1
- TNF
ASJC Scopus subject areas
- Endocrinology
- Molecular Biology
- Immunology
- Immunology and Allergy
Cite this
Interleukin 8 and monocyte chemoattractant protein 1 production by cultured human airway smooth muscle cells. / Watson, Malcolm L.; Grix, Suzanna P.; Jordan, Nicola J.; Place, Graham A.; Dodd, Sara; Leithead, Jane; Poll, Christopher T.; Yoshimura, Teizo; Westwick, John.
In: Cytokine, Vol. 10, No. 5, 05.1998, p. 346-352.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interleukin 8 and monocyte chemoattractant protein 1 production by cultured human airway smooth muscle cells
AU - Watson, Malcolm L.
AU - Grix, Suzanna P.
AU - Jordan, Nicola J.
AU - Place, Graham A.
AU - Dodd, Sara
AU - Leithead, Jane
AU - Poll, Christopher T.
AU - Yoshimura, Teizo
AU - Westwick, John
PY - 1998/5
Y1 - 1998/5
N2 - Leukocyte accumulation and activation are key events in the pathogenesis of inflammatory lung disease. The ability of human airway smooth muscle cells (HASM) to contribute to the inflammatory process by its ability to produce the chemokines interleukin (IL) 8, monocyte chemotactic protein (MCP-1) and regulated on activation, normal T cell expressed and secreted (RANTES) was investigated. Cultured HASM, when stimulated with the pro-inflammatory cytokines IL-1α (0.01-2 ng/ml) or tumour necrosis factor or (TNF-α, 0.3-30 ng/ml), synthesize and release substantial amounts of IL-8, as assessed by specific immunoassay, bioasssay (elevation of intracellular free calcium in human neutrophils), and upregulation of mRNA. These stimuli also increased MCP-1 production and mRNA expression, but RANTES mRNA expression was not detected at 24 h. The smooth muscle spasmogen endothelin 1 (1 μM) was unable to stimulate IL-8 or MCP-1 release or mRNA expression. These data indicate that HASM may constitute an important source of leukocyte attractants in the inflamed lung, where the inducing stimuli, IL-1α and TNF-α, are also likely to be present.
AB - Leukocyte accumulation and activation are key events in the pathogenesis of inflammatory lung disease. The ability of human airway smooth muscle cells (HASM) to contribute to the inflammatory process by its ability to produce the chemokines interleukin (IL) 8, monocyte chemotactic protein (MCP-1) and regulated on activation, normal T cell expressed and secreted (RANTES) was investigated. Cultured HASM, when stimulated with the pro-inflammatory cytokines IL-1α (0.01-2 ng/ml) or tumour necrosis factor or (TNF-α, 0.3-30 ng/ml), synthesize and release substantial amounts of IL-8, as assessed by specific immunoassay, bioasssay (elevation of intracellular free calcium in human neutrophils), and upregulation of mRNA. These stimuli also increased MCP-1 production and mRNA expression, but RANTES mRNA expression was not detected at 24 h. The smooth muscle spasmogen endothelin 1 (1 μM) was unable to stimulate IL-8 or MCP-1 release or mRNA expression. These data indicate that HASM may constitute an important source of leukocyte attractants in the inflamed lung, where the inducing stimuli, IL-1α and TNF-α, are also likely to be present.
KW - Airway smooth muscle
KW - Chemokines
KW - IL-1
KW - TNF
UR - http://www.scopus.com/inward/record.url?scp=17144448563&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17144448563&partnerID=8YFLogxK
U2 - 10.1006/cyto.1997.0350
DO - 10.1006/cyto.1997.0350
M3 - Article
C2 - 9619372
AN - SCOPUS:17144448563
VL - 10
SP - 346
EP - 352
JO - Cytokine
JF - Cytokine
SN - 1043-4666
IS - 5
ER -