Interleukin 8 and monocyte chemoattractant protein 1 production by cultured human airway smooth muscle cells

Malcolm L. Watson, Suzanna P. Grix, Nicola J. Jordan, Graham A. Place, Sara Dodd, Jane Leithead, Christopher T. Poll, Teizo Yoshimura, John Westwick

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Leukocyte accumulation and activation are key events in the pathogenesis of inflammatory lung disease. The ability of human airway smooth muscle cells (HASM) to contribute to the inflammatory process by its ability to produce the chemokines interleukin (IL) 8, monocyte chemotactic protein (MCP-1) and regulated on activation, normal T cell expressed and secreted (RANTES) was investigated. Cultured HASM, when stimulated with the pro-inflammatory cytokines IL-1α (0.01-2 ng/ml) or tumour necrosis factor or (TNF-α, 0.3-30 ng/ml), synthesize and release substantial amounts of IL-8, as assessed by specific immunoassay, bioasssay (elevation of intracellular free calcium in human neutrophils), and upregulation of mRNA. These stimuli also increased MCP-1 production and mRNA expression, but RANTES mRNA expression was not detected at 24 h. The smooth muscle spasmogen endothelin 1 (1 μM) was unable to stimulate IL-8 or MCP-1 release or mRNA expression. These data indicate that HASM may constitute an important source of leukocyte attractants in the inflamed lung, where the inducing stimuli, IL-1α and TNF-α, are also likely to be present.

Original languageEnglish
Pages (from-to)346-352
Number of pages7
JournalCytokine
Volume10
Issue number5
DOIs
Publication statusPublished - May 1998
Externally publishedYes

Fingerprint

Chemokine CCL2
Interleukin-8
Smooth Muscle Myocytes
Muscle
Cells
Messenger RNA
T-cells
Chemical activation
Interleukin-1
Monocyte Chemoattractant Proteins
Leukocytes
T-Lymphocytes
Pulmonary diseases
Endothelin-1
Chemokines
Immunoassay
Lung Diseases
Smooth Muscle
Neutrophils
Up-Regulation

Keywords

  • Airway smooth muscle
  • Chemokines
  • IL-1
  • TNF

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Watson, M. L., Grix, S. P., Jordan, N. J., Place, G. A., Dodd, S., Leithead, J., ... Westwick, J. (1998). Interleukin 8 and monocyte chemoattractant protein 1 production by cultured human airway smooth muscle cells. Cytokine, 10(5), 346-352. https://doi.org/10.1006/cyto.1997.0350

Interleukin 8 and monocyte chemoattractant protein 1 production by cultured human airway smooth muscle cells. / Watson, Malcolm L.; Grix, Suzanna P.; Jordan, Nicola J.; Place, Graham A.; Dodd, Sara; Leithead, Jane; Poll, Christopher T.; Yoshimura, Teizo; Westwick, John.

In: Cytokine, Vol. 10, No. 5, 05.1998, p. 346-352.

Research output: Contribution to journalArticle

Watson, ML, Grix, SP, Jordan, NJ, Place, GA, Dodd, S, Leithead, J, Poll, CT, Yoshimura, T & Westwick, J 1998, 'Interleukin 8 and monocyte chemoattractant protein 1 production by cultured human airway smooth muscle cells', Cytokine, vol. 10, no. 5, pp. 346-352. https://doi.org/10.1006/cyto.1997.0350
Watson, Malcolm L. ; Grix, Suzanna P. ; Jordan, Nicola J. ; Place, Graham A. ; Dodd, Sara ; Leithead, Jane ; Poll, Christopher T. ; Yoshimura, Teizo ; Westwick, John. / Interleukin 8 and monocyte chemoattractant protein 1 production by cultured human airway smooth muscle cells. In: Cytokine. 1998 ; Vol. 10, No. 5. pp. 346-352.
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