Interleukin-4-mediated suppression of histamine synthesis in a murine mast cell line, BNu-2cl3

Norio Sato, Satoshi Tanaka, Junji Matsuura, Yuhji Takasu, Atsushi Ichikawa

Research output: Contribution to journalArticle

Abstract

Since a wide variety of heterogeneity was found in tissue mast cells, recent studies have focused on the process of differentiation and maturation of mast cells. It has been largely accepted that the ability of histamine synthesis is high in the mucosal type mast cells whereas that is low in the tissue-connective type mast cells, although it remains largely unknown how histamine synthesis is regulated during differentiation. Interleukin (IL)-4 is one of the candidate factors that regulate the process of mast cell differentiation. We investigated the effects of IL-4 on histamine synthesis using a murine IL-3-dependent mucosal-type mast cell line, BNu-2cl3. IL-4 drastically suppressed histamine synthesis at the transcriptional levels. Storage of histamine was significantly decreased upon prolonged treatment with IL-4. Down-regulation in expression of histidine decarboxylase by IL-4 was restored by addition of excessive amount of IL-3. Changes in mRNA expression of mouse mast cell proteases (MMCPs) in the cells treated with IL-4 mimicked the differentiation process from mucosal-type to connective tissue-type mast cells; mRNA expression of MMCP2 was decreased, whereas that of MMCP4 and carboxypeptidase A3 were unchanged. These results suggest that IL-4 should play a critical role in suppression of histamine synthesis in mucosal-type mast cells.

Original languageEnglish
Pages (from-to)1800-1802
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume32
Issue number10
DOIs
Publication statusPublished - Oct 2009
Externally publishedYes

Fingerprint

Mast Cells
Interleukin-4
Histamine
Cell Line
Interleukin-3
Histidine Decarboxylase
Connective Tissue Cells
Carboxypeptidases
Messenger RNA
Connective Tissue
Cell Differentiation
Peptide Hydrolases
Down-Regulation

Keywords

  • Histamine
  • Histidine decarboxylase
  • Interluekin-4
  • Mast cell

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Interleukin-4-mediated suppression of histamine synthesis in a murine mast cell line, BNu-2cl3. / Sato, Norio; Tanaka, Satoshi; Matsuura, Junji; Takasu, Yuhji; Ichikawa, Atsushi.

In: Biological and Pharmaceutical Bulletin, Vol. 32, No. 10, 10.2009, p. 1800-1802.

Research output: Contribution to journalArticle

Sato, Norio ; Tanaka, Satoshi ; Matsuura, Junji ; Takasu, Yuhji ; Ichikawa, Atsushi. / Interleukin-4-mediated suppression of histamine synthesis in a murine mast cell line, BNu-2cl3. In: Biological and Pharmaceutical Bulletin. 2009 ; Vol. 32, No. 10. pp. 1800-1802.
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