Interleukin-4-independent production of Th2 cytokines by nasal lymphocytes and nasal eosinophilia in murine allergic rhinitis

M. Okano, A. R. Satoskar, M. Abe, D. A. Harn, Kazunori Nishizaki, Y. Takeda, Tadashi Yoshino, F. Brombacher, A. A. Satoskar

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background: Interleukin (IL)-4 is believed to play an important role in the atopic pathogenesis. However, the precise role of IL-4 in the in vivo initiation of allergic rhinitis is not fully understood. We have recently found that BALB/c mice sensitized intranasally with Schistosoma mansoni egg antigen (SEA) mount a Th2 response that initiates allergic rhinitis. Thus, we sought to determine the role of IL-4 in the initiation of allergic rhinitis in vivo with this model. Methods: IL-4 gene-deficient (IL-4-/-) BALB/c and wild-type (IL-4 +/+) control mice were sensitized by intranasal SEA administration, and their immunologic responses were examined both in vivo and in vitro. Results: IL-4+/+ mice sensitized with SEA displayed significantly higher titers of SEA-specific IgG1 and IgE antibodies than IL- 4-/- mice, while the latter produced significantly more SEA-specific IgG2a. Antigen-stimulated nasal lymphocytes from SEA-sensitized IL-4-/- and IL-4+/+ mice produced similar amounts of IL-5 and IL-10, but neither produced IFN-γ. Furthermore, the severity of nasal eosinophilia was similar in both groups. Conclusions: These results indicate that although IL-4 is necessary for the production of Th2-associated antibodies - in particular, IgE - it is not required for either the production of the Th2-associated cytokines IL-5 and IL-10, or the induction of nasal eosinophilia.

Original languageEnglish
Pages (from-to)723-731
Number of pages9
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume55
Issue number8
DOIs
Publication statusPublished - 2000

Fingerprint

Eosinophilia
Nose
Interleukin-4
Lymphocytes
Schistosoma mansoni
Cytokines
Ovum
Antigens
Interleukin-5
Interleukin-10
Immunoglobulin E
Allergic Rhinitis
Antibodies
Immunoglobulin G

Keywords

  • Allergic rhinitis
  • Eosinophil
  • IgE
  • IL-4
  • IL-5
  • Mouse

ASJC Scopus subject areas

  • Immunology

Cite this

Interleukin-4-independent production of Th2 cytokines by nasal lymphocytes and nasal eosinophilia in murine allergic rhinitis. / Okano, M.; Satoskar, A. R.; Abe, M.; Harn, D. A.; Nishizaki, Kazunori; Takeda, Y.; Yoshino, Tadashi; Brombacher, F.; Satoskar, A. A.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 55, No. 8, 2000, p. 723-731.

Research output: Contribution to journalArticle

@article{b5a1e2452e5d441895703905fc5c843b,
title = "Interleukin-4-independent production of Th2 cytokines by nasal lymphocytes and nasal eosinophilia in murine allergic rhinitis",
abstract = "Background: Interleukin (IL)-4 is believed to play an important role in the atopic pathogenesis. However, the precise role of IL-4 in the in vivo initiation of allergic rhinitis is not fully understood. We have recently found that BALB/c mice sensitized intranasally with Schistosoma mansoni egg antigen (SEA) mount a Th2 response that initiates allergic rhinitis. Thus, we sought to determine the role of IL-4 in the initiation of allergic rhinitis in vivo with this model. Methods: IL-4 gene-deficient (IL-4-/-) BALB/c and wild-type (IL-4 +/+) control mice were sensitized by intranasal SEA administration, and their immunologic responses were examined both in vivo and in vitro. Results: IL-4+/+ mice sensitized with SEA displayed significantly higher titers of SEA-specific IgG1 and IgE antibodies than IL- 4-/- mice, while the latter produced significantly more SEA-specific IgG2a. Antigen-stimulated nasal lymphocytes from SEA-sensitized IL-4-/- and IL-4+/+ mice produced similar amounts of IL-5 and IL-10, but neither produced IFN-γ. Furthermore, the severity of nasal eosinophilia was similar in both groups. Conclusions: These results indicate that although IL-4 is necessary for the production of Th2-associated antibodies - in particular, IgE - it is not required for either the production of the Th2-associated cytokines IL-5 and IL-10, or the induction of nasal eosinophilia.",
keywords = "Allergic rhinitis, Eosinophil, IgE, IL-4, IL-5, Mouse",
author = "M. Okano and Satoskar, {A. R.} and M. Abe and Harn, {D. A.} and Kazunori Nishizaki and Y. Takeda and Tadashi Yoshino and F. Brombacher and Satoskar, {A. A.}",
year = "2000",
doi = "10.1034/j.1398-9995.2000.00429.x",
language = "English",
volume = "55",
pages = "723--731",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
issn = "0105-4538",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - Interleukin-4-independent production of Th2 cytokines by nasal lymphocytes and nasal eosinophilia in murine allergic rhinitis

AU - Okano, M.

AU - Satoskar, A. R.

AU - Abe, M.

AU - Harn, D. A.

AU - Nishizaki, Kazunori

AU - Takeda, Y.

AU - Yoshino, Tadashi

AU - Brombacher, F.

AU - Satoskar, A. A.

PY - 2000

Y1 - 2000

N2 - Background: Interleukin (IL)-4 is believed to play an important role in the atopic pathogenesis. However, the precise role of IL-4 in the in vivo initiation of allergic rhinitis is not fully understood. We have recently found that BALB/c mice sensitized intranasally with Schistosoma mansoni egg antigen (SEA) mount a Th2 response that initiates allergic rhinitis. Thus, we sought to determine the role of IL-4 in the initiation of allergic rhinitis in vivo with this model. Methods: IL-4 gene-deficient (IL-4-/-) BALB/c and wild-type (IL-4 +/+) control mice were sensitized by intranasal SEA administration, and their immunologic responses were examined both in vivo and in vitro. Results: IL-4+/+ mice sensitized with SEA displayed significantly higher titers of SEA-specific IgG1 and IgE antibodies than IL- 4-/- mice, while the latter produced significantly more SEA-specific IgG2a. Antigen-stimulated nasal lymphocytes from SEA-sensitized IL-4-/- and IL-4+/+ mice produced similar amounts of IL-5 and IL-10, but neither produced IFN-γ. Furthermore, the severity of nasal eosinophilia was similar in both groups. Conclusions: These results indicate that although IL-4 is necessary for the production of Th2-associated antibodies - in particular, IgE - it is not required for either the production of the Th2-associated cytokines IL-5 and IL-10, or the induction of nasal eosinophilia.

AB - Background: Interleukin (IL)-4 is believed to play an important role in the atopic pathogenesis. However, the precise role of IL-4 in the in vivo initiation of allergic rhinitis is not fully understood. We have recently found that BALB/c mice sensitized intranasally with Schistosoma mansoni egg antigen (SEA) mount a Th2 response that initiates allergic rhinitis. Thus, we sought to determine the role of IL-4 in the initiation of allergic rhinitis in vivo with this model. Methods: IL-4 gene-deficient (IL-4-/-) BALB/c and wild-type (IL-4 +/+) control mice were sensitized by intranasal SEA administration, and their immunologic responses were examined both in vivo and in vitro. Results: IL-4+/+ mice sensitized with SEA displayed significantly higher titers of SEA-specific IgG1 and IgE antibodies than IL- 4-/- mice, while the latter produced significantly more SEA-specific IgG2a. Antigen-stimulated nasal lymphocytes from SEA-sensitized IL-4-/- and IL-4+/+ mice produced similar amounts of IL-5 and IL-10, but neither produced IFN-γ. Furthermore, the severity of nasal eosinophilia was similar in both groups. Conclusions: These results indicate that although IL-4 is necessary for the production of Th2-associated antibodies - in particular, IgE - it is not required for either the production of the Th2-associated cytokines IL-5 and IL-10, or the induction of nasal eosinophilia.

KW - Allergic rhinitis

KW - Eosinophil

KW - IgE

KW - IL-4

KW - IL-5

KW - Mouse

UR - http://www.scopus.com/inward/record.url?scp=0033860548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033860548&partnerID=8YFLogxK

U2 - 10.1034/j.1398-9995.2000.00429.x

DO - 10.1034/j.1398-9995.2000.00429.x

M3 - Article

VL - 55

SP - 723

EP - 731

JO - Allergy: European Journal of Allergy and Clinical Immunology

JF - Allergy: European Journal of Allergy and Clinical Immunology

SN - 0105-4538

IS - 8

ER -