Interleukin 34 (IL-34) cell-surface localization regulated by the molecular chaperone 78-kDa glucose-regulated protein facilitates the differentiation of monocytic cells

Sayaka Ogawa, Yukiko Matsuoka, Miho Takada, Kazue Matsui, Fumihiro Yamane, Eri Kubota, Shiori Yasuhara, Kentaro Hieda, Naoki Kanayama, Naoya Hatano, Hiroshi Tokumitsu, Masaki Magari

Research output: Contribution to journalArticle

Abstract

Interleukin 34 (IL-34) constitutes a cytokine that shares a common receptor, colony-stimulating factor-1 receptor (CSF-1R), with CSF-1. We recently identified a novel type of monocytic cell termed follicular dendritic cell-induced monocytic cells (FDMCs), whose differentiation depended on CSF-1R signaling through the IL-34 produced from a follicular dendritic cell line, FL-Y. Here, we report the functional mechanisms of the IL-34 –mediated CSF-1R signaling underlying FDMC differentiation. CRIPSR/Cas9-mediated knockout of the Il34 gene confirmed that the ability of FL-Y cells to induce FDMCs completely depends on the IL-34 expressed by FL-Y cells. Transwell culture experiments revealed that FDMC differentiation requires a signal from a membrane-anchored form of IL-34 on the FL-Y cell surface, but not from a secreted form, in a direct interaction between FDMC precursor cells and FL-Y cells. Furthermore, flow cytometric analysis using an anti–IL-34 antibody indicated that IL-34 was also expressed on the FL-Y cell surface. Thus, we explored proteins interacting with IL-34 in FL-Y cells. Mass spectrometry analysis and pulldown assay identified that IL-34 was associated with the molecular chaperone 78-kDa glucose-regulated protein (GRP78) in the plasma membrane fraction of FL-Y cells. Consistent with this finding, GRP78-heterozygous FL-Y cells expressed a lower level of IL-34 protein on their cell surface and exhibited a reduced competency to induce FDMC differentiation compared with the original FL-Y cells. These results indicated a novel GRP78-dependent localization and specific function of IL-34 in FL-Y cells related to monocytic cell differentiation.

Original languageEnglish
Pages (from-to)2386-2396
Number of pages11
JournalJournal of Biological Chemistry
Volume294
Issue number7
DOIs
Publication statusPublished - Jan 1 2019

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Molecular Chaperones
Interleukins
Cell Differentiation
Cells
Macrophage Colony-Stimulating Factor
Colony-Stimulating Factor Receptors
Follicular Dendritic Cells
molecular chaperone GRP78
Cell membranes
Mass spectrometry
Gene Knockout Techniques
Assays
Proteins
Genes
Cytokines
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Interleukin 34 (IL-34) cell-surface localization regulated by the molecular chaperone 78-kDa glucose-regulated protein facilitates the differentiation of monocytic cells. / Ogawa, Sayaka; Matsuoka, Yukiko; Takada, Miho; Matsui, Kazue; Yamane, Fumihiro; Kubota, Eri; Yasuhara, Shiori; Hieda, Kentaro; Kanayama, Naoki; Hatano, Naoya; Tokumitsu, Hiroshi; Magari, Masaki.

In: Journal of Biological Chemistry, Vol. 294, No. 7, 01.01.2019, p. 2386-2396.

Research output: Contribution to journalArticle

Ogawa, Sayaka ; Matsuoka, Yukiko ; Takada, Miho ; Matsui, Kazue ; Yamane, Fumihiro ; Kubota, Eri ; Yasuhara, Shiori ; Hieda, Kentaro ; Kanayama, Naoki ; Hatano, Naoya ; Tokumitsu, Hiroshi ; Magari, Masaki. / Interleukin 34 (IL-34) cell-surface localization regulated by the molecular chaperone 78-kDa glucose-regulated protein facilitates the differentiation of monocytic cells. In: Journal of Biological Chemistry. 2019 ; Vol. 294, No. 7. pp. 2386-2396.
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AU - Ogawa, Sayaka

AU - Matsuoka, Yukiko

AU - Takada, Miho

AU - Matsui, Kazue

AU - Yamane, Fumihiro

AU - Kubota, Eri

AU - Yasuhara, Shiori

AU - Hieda, Kentaro

AU - Kanayama, Naoki

AU - Hatano, Naoya

AU - Tokumitsu, Hiroshi

AU - Magari, Masaki

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AB - Interleukin 34 (IL-34) constitutes a cytokine that shares a common receptor, colony-stimulating factor-1 receptor (CSF-1R), with CSF-1. We recently identified a novel type of monocytic cell termed follicular dendritic cell-induced monocytic cells (FDMCs), whose differentiation depended on CSF-1R signaling through the IL-34 produced from a follicular dendritic cell line, FL-Y. Here, we report the functional mechanisms of the IL-34 –mediated CSF-1R signaling underlying FDMC differentiation. CRIPSR/Cas9-mediated knockout of the Il34 gene confirmed that the ability of FL-Y cells to induce FDMCs completely depends on the IL-34 expressed by FL-Y cells. Transwell culture experiments revealed that FDMC differentiation requires a signal from a membrane-anchored form of IL-34 on the FL-Y cell surface, but not from a secreted form, in a direct interaction between FDMC precursor cells and FL-Y cells. Furthermore, flow cytometric analysis using an anti–IL-34 antibody indicated that IL-34 was also expressed on the FL-Y cell surface. Thus, we explored proteins interacting with IL-34 in FL-Y cells. Mass spectrometry analysis and pulldown assay identified that IL-34 was associated with the molecular chaperone 78-kDa glucose-regulated protein (GRP78) in the plasma membrane fraction of FL-Y cells. Consistent with this finding, GRP78-heterozygous FL-Y cells expressed a lower level of IL-34 protein on their cell surface and exhibited a reduced competency to induce FDMC differentiation compared with the original FL-Y cells. These results indicated a novel GRP78-dependent localization and specific function of IL-34 in FL-Y cells related to monocytic cell differentiation.

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