Interleukin-18/interferon-γ-inducing factor, a novel cytokine, up- regulates ICAM-1 (CD54) expression in KG-1 cells

Hideo Kohka, Tadashi Yoshino, Hiromi Iwagaki, Isao Sakuma, Tadao Tanimoto, Yosinobu Matsuo, Masashi Kurimoto, Kunzo Orita, Tadaatsu Akagi, Noriaki Tanaka

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Intercellular adhesion molecule-1 (ICAM-1, CD54) is a membrane glycoprotein and a member of the immunoglobulin superfamily. It plays a central role in cell to cell-mediated immune responses and is a ligand for leukocyte function-associated antigen-1 (LFA-1). We report here that a newly discovered cytokine, interferon-γ-inducing factor (IGIF) [H. Okamura et al. (1995) Nature 378, 88] recently proposed to be designated as IL-18, selectively up-regulates ICAM-1 expression in KG-1 cells, a human myelomonocytic cell line, in which IL-18 also enhances interferon-γ production. IL-18 induced heterotypic aggregation between KG-1 and Peer T cells, which was blocked by anti-ICAM-1 and/or LFA-1 antibodies. Anti- interferon-γ antibody did not block the IL-18-induced up-regulation of ICAM- 1 on KG-1 cells. These results thus show that IGIF/IL-18, enhances ICAM-1 expression in KG-1 cells in an interferon-γ-independent pathway, up- regulates ICAM-1 functions, and that IL-18 might play a potential role in immunoregulation by mediating immune cell infiltration into the tissues.

Original languageEnglish
Pages (from-to)519-527
Number of pages9
JournalJournal of Leukocyte Biology
Volume64
Issue number4
Publication statusPublished - Oct 1998

Fingerprint

Interleukin-18
Intercellular Adhesion Molecule-1
Interferons
Up-Regulation
Cytokines
Lymphocyte Function-Associated Antigen-1
Membrane Glycoproteins
Immunoglobulins
Anti-Idiotypic Antibodies
Ligands
T-Lymphocytes
Cell Line
Antibodies

Keywords

  • Aggregation
  • Cytokine
  • Flow cytometry
  • Reverse transcriptase-polymerase chain reaction

ASJC Scopus subject areas

  • Cell Biology

Cite this

Kohka, H., Yoshino, T., Iwagaki, H., Sakuma, I., Tanimoto, T., Matsuo, Y., ... Tanaka, N. (1998). Interleukin-18/interferon-γ-inducing factor, a novel cytokine, up- regulates ICAM-1 (CD54) expression in KG-1 cells. Journal of Leukocyte Biology, 64(4), 519-527.

Interleukin-18/interferon-γ-inducing factor, a novel cytokine, up- regulates ICAM-1 (CD54) expression in KG-1 cells. / Kohka, Hideo; Yoshino, Tadashi; Iwagaki, Hiromi; Sakuma, Isao; Tanimoto, Tadao; Matsuo, Yosinobu; Kurimoto, Masashi; Orita, Kunzo; Akagi, Tadaatsu; Tanaka, Noriaki.

In: Journal of Leukocyte Biology, Vol. 64, No. 4, 10.1998, p. 519-527.

Research output: Contribution to journalArticle

Kohka, H, Yoshino, T, Iwagaki, H, Sakuma, I, Tanimoto, T, Matsuo, Y, Kurimoto, M, Orita, K, Akagi, T & Tanaka, N 1998, 'Interleukin-18/interferon-γ-inducing factor, a novel cytokine, up- regulates ICAM-1 (CD54) expression in KG-1 cells', Journal of Leukocyte Biology, vol. 64, no. 4, pp. 519-527.
Kohka, Hideo ; Yoshino, Tadashi ; Iwagaki, Hiromi ; Sakuma, Isao ; Tanimoto, Tadao ; Matsuo, Yosinobu ; Kurimoto, Masashi ; Orita, Kunzo ; Akagi, Tadaatsu ; Tanaka, Noriaki. / Interleukin-18/interferon-γ-inducing factor, a novel cytokine, up- regulates ICAM-1 (CD54) expression in KG-1 cells. In: Journal of Leukocyte Biology. 1998 ; Vol. 64, No. 4. pp. 519-527.
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