Interleukin-18 amplifies macrophage polarization and morphological alteration, leading to excessive angiogenesis

Takuro Kobori, Shinichi Hamasaki, Atsuhiro Kitaura, Yui Yamazaki, Takashi Nishinaka, Atsuko Niwa, Shinichi Nakao, Hidenori Wake, Shuji Mori, Tadashi Yoshino, Masahiro Nishibori, Hideo Takahashi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

M2 macrophage (Mψ) promotes pathologic angiogenesis through a release of pro-angiogenic mediators or the direct cell-cell interaction with endothelium in the micromilieu of several chronic inflammatory diseases, including rheumatoid arthritis and cancer, where interleukin (IL)-18 also contributes to excessive angiogenesis. However, the detailed mechanism remains unclear. The aim of this study is to investigate the mechanism by which M2 Mψs in the micromilieu containing IL-18 induce excessive angiogenesis in the in vitro experimental model using mouse Mψ-like cell line, RAW264.7 cells, and mouse endothelial cell line, b. End5 cells. We discovered that IL-18 acts synergistically with IL-10 to amplify the production of Mψ-derived mediators like osteopontin (OPN) and thrombin, yielding thrombin-cleaved form of OPN generation, which acts through integrins α4/α9, thereby augmenting M2 polarization of Mψ with characteristics of increasing surface CD163 expression in association with morphological alteration. Furthermore, the results of visualizing temporal behavior and morphological alteration of Mψs during angiogenesis demonstrated that M2-like Mψs induced excessive angiogenesis through the direct cell-cell interaction with endothelial cells, possibly mediated by CD163.

Original languageEnglish
Article number334
JournalFrontiers in Immunology
Volume9
Issue numberMAR
DOIs
Publication statusPublished - Mar 6 2018

Fingerprint

Interleukin-18
Macrophages
Osteopontin
Thrombin
Cell Communication
Endothelial Cells
Pathologic Neovascularization
Cell Line
Integrins
Interleukin-10
Endothelium
Rheumatoid Arthritis
Chronic Disease
Theoretical Models
Neoplasms

Keywords

  • Angiogenesis
  • CD163
  • Interleukin-18
  • Macrophage
  • Osteopontin
  • Thrombin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Kobori, T., Hamasaki, S., Kitaura, A., Yamazaki, Y., Nishinaka, T., Niwa, A., ... Takahashi, H. (2018). Interleukin-18 amplifies macrophage polarization and morphological alteration, leading to excessive angiogenesis. Frontiers in Immunology, 9(MAR), [334]. https://doi.org/10.3389/fimmu.2018.00334

Interleukin-18 amplifies macrophage polarization and morphological alteration, leading to excessive angiogenesis. / Kobori, Takuro; Hamasaki, Shinichi; Kitaura, Atsuhiro; Yamazaki, Yui; Nishinaka, Takashi; Niwa, Atsuko; Nakao, Shinichi; Wake, Hidenori; Mori, Shuji; Yoshino, Tadashi; Nishibori, Masahiro; Takahashi, Hideo.

In: Frontiers in Immunology, Vol. 9, No. MAR, 334, 06.03.2018.

Research output: Contribution to journalArticle

Kobori, Takuro ; Hamasaki, Shinichi ; Kitaura, Atsuhiro ; Yamazaki, Yui ; Nishinaka, Takashi ; Niwa, Atsuko ; Nakao, Shinichi ; Wake, Hidenori ; Mori, Shuji ; Yoshino, Tadashi ; Nishibori, Masahiro ; Takahashi, Hideo. / Interleukin-18 amplifies macrophage polarization and morphological alteration, leading to excessive angiogenesis. In: Frontiers in Immunology. 2018 ; Vol. 9, No. MAR.
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