Interleukin-15 is not a constitutive cytokine in the epidermis, but is inducible in culture or inflammatory conditions

Gang Wen Han, Keiji Iwatsuki, Masayuki Inoue, Takashi Matsui, Akiko Nishibu, Hitoshi Akiba, Fumio Kaneko

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The regulation in the skin of interleukin-15 (IL-15), a potent modulator of T-cell-mediated immune responses, is not fully understood. We investigated the levels of IL-15 and its mRNA produced by epidermal and cultured keratinocytes and found that normal keratinocytes did not constitutively express IL-15 in the epidermis, but in culture began to produce the cytokine. Some epidermal keratinocytes expressed IL-15 in inflammatory conditions associated with infiltration of neutrophils and eosinophils. IL-15 was detected only in the cell lysates, not in the supernatants of cultured keratinocytes. Dexamethasone (10-5- 10-6 M) markedly inhibited IL-15 mRNA expression by normal and transformed keratinocytes in a range of pharmacological concentrations. IFN-(γ) (200 and 400 U/ml) slightly increased the 1L-15 message level in a squamous cell carcinoma cell line, HSC-5, in a dose-dependent fashion, whereas no significant change was observed in cultured normal human keratinocytes. Our data indicate that IL- 15 is not a constitutive cytokine in epidermal keratinocytes but is inducible.

Original languageEnglish
Pages (from-to)37-40
Number of pages4
JournalActa Dermato-Venereologica
Volume79
Issue number1
DOIs
Publication statusPublished - 1999
Externally publishedYes

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Interleukin-15
Keratinocytes
Epidermis
Cytokines
Messenger RNA
Neutrophil Infiltration
Eosinophils
Dexamethasone
Squamous Cell Carcinoma
Pharmacology
T-Lymphocytes
Cell Line
Skin

Keywords

  • Dexamethasone
  • Human squamous cell carcinoma
  • IL-15
  • Interferon-γ
  • Keratinocyte
  • Northern blotting

ASJC Scopus subject areas

  • Dermatology

Cite this

Interleukin-15 is not a constitutive cytokine in the epidermis, but is inducible in culture or inflammatory conditions. / Han, Gang Wen; Iwatsuki, Keiji; Inoue, Masayuki; Matsui, Takashi; Nishibu, Akiko; Akiba, Hitoshi; Kaneko, Fumio.

In: Acta Dermato-Venereologica, Vol. 79, No. 1, 1999, p. 37-40.

Research output: Contribution to journalArticle

Han, Gang Wen ; Iwatsuki, Keiji ; Inoue, Masayuki ; Matsui, Takashi ; Nishibu, Akiko ; Akiba, Hitoshi ; Kaneko, Fumio. / Interleukin-15 is not a constitutive cytokine in the epidermis, but is inducible in culture or inflammatory conditions. In: Acta Dermato-Venereologica. 1999 ; Vol. 79, No. 1. pp. 37-40.
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