Abstract
The regulation in the skin of interleukin-15 (IL-15), a potent modulator of T-cell-mediated immune responses, is not fully understood. We investigated the levels of IL-15 and its mRNA produced by epidermal and cultured keratinocytes and found that normal keratinocytes did not constitutively express IL-15 in the epidermis, but in culture began to produce the cytokine. Some epidermal keratinocytes expressed IL-15 in inflammatory conditions associated with infiltration of neutrophils and eosinophils. IL-15 was detected only in the cell lysates, not in the supernatants of cultured keratinocytes. Dexamethasone (10-5- 10-6 M) markedly inhibited IL-15 mRNA expression by normal and transformed keratinocytes in a range of pharmacological concentrations. IFN-(γ) (200 and 400 U/ml) slightly increased the 1L-15 message level in a squamous cell carcinoma cell line, HSC-5, in a dose-dependent fashion, whereas no significant change was observed in cultured normal human keratinocytes. Our data indicate that IL- 15 is not a constitutive cytokine in epidermal keratinocytes but is inducible.
| Original language | English |
|---|---|
| Pages (from-to) | 37-40 |
| Number of pages | 4 |
| Journal | Acta Dermato-Venereologica |
| Volume | 79 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1999 |
| Externally published | Yes |
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Keywords
- Dexamethasone
- Human squamous cell carcinoma
- IL-15
- Interferon-γ
- Keratinocyte
- Northern blotting
ASJC Scopus subject areas
- Dermatology
Cite this
Interleukin-15 is not a constitutive cytokine in the epidermis, but is inducible in culture or inflammatory conditions. / Han, Gang Wen; Iwatsuki, Keiji; Inoue, Masayuki; Matsui, Takashi; Nishibu, Akiko; Akiba, Hitoshi; Kaneko, Fumio.
In: Acta Dermato-Venereologica, Vol. 79, No. 1, 1999, p. 37-40.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interleukin-15 is not a constitutive cytokine in the epidermis, but is inducible in culture or inflammatory conditions
AU - Han, Gang Wen
AU - Iwatsuki, Keiji
AU - Inoue, Masayuki
AU - Matsui, Takashi
AU - Nishibu, Akiko
AU - Akiba, Hitoshi
AU - Kaneko, Fumio
PY - 1999
Y1 - 1999
N2 - The regulation in the skin of interleukin-15 (IL-15), a potent modulator of T-cell-mediated immune responses, is not fully understood. We investigated the levels of IL-15 and its mRNA produced by epidermal and cultured keratinocytes and found that normal keratinocytes did not constitutively express IL-15 in the epidermis, but in culture began to produce the cytokine. Some epidermal keratinocytes expressed IL-15 in inflammatory conditions associated with infiltration of neutrophils and eosinophils. IL-15 was detected only in the cell lysates, not in the supernatants of cultured keratinocytes. Dexamethasone (10-5- 10-6 M) markedly inhibited IL-15 mRNA expression by normal and transformed keratinocytes in a range of pharmacological concentrations. IFN-(γ) (200 and 400 U/ml) slightly increased the 1L-15 message level in a squamous cell carcinoma cell line, HSC-5, in a dose-dependent fashion, whereas no significant change was observed in cultured normal human keratinocytes. Our data indicate that IL- 15 is not a constitutive cytokine in epidermal keratinocytes but is inducible.
AB - The regulation in the skin of interleukin-15 (IL-15), a potent modulator of T-cell-mediated immune responses, is not fully understood. We investigated the levels of IL-15 and its mRNA produced by epidermal and cultured keratinocytes and found that normal keratinocytes did not constitutively express IL-15 in the epidermis, but in culture began to produce the cytokine. Some epidermal keratinocytes expressed IL-15 in inflammatory conditions associated with infiltration of neutrophils and eosinophils. IL-15 was detected only in the cell lysates, not in the supernatants of cultured keratinocytes. Dexamethasone (10-5- 10-6 M) markedly inhibited IL-15 mRNA expression by normal and transformed keratinocytes in a range of pharmacological concentrations. IFN-(γ) (200 and 400 U/ml) slightly increased the 1L-15 message level in a squamous cell carcinoma cell line, HSC-5, in a dose-dependent fashion, whereas no significant change was observed in cultured normal human keratinocytes. Our data indicate that IL- 15 is not a constitutive cytokine in epidermal keratinocytes but is inducible.
KW - Dexamethasone
KW - Human squamous cell carcinoma
KW - IL-15
KW - Interferon-γ
KW - Keratinocyte
KW - Northern blotting
UR - http://www.scopus.com/inward/record.url?scp=0032990971&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032990971&partnerID=8YFLogxK
U2 - 10.1080/000155599750011679
DO - 10.1080/000155599750011679
M3 - Article
C2 - 10086856
AN - SCOPUS:0032990971
VL - 79
SP - 37
EP - 40
JO - Acta Dermato-Venereologica
JF - Acta Dermato-Venereologica
SN - 0001-5555
IS - 1
ER -