Abstract
The aim was to investigate joint perfusate levels of interleukin-1β (1L-1β) in antigen-induced monoarthritis of the rabbit temporomandibular (TMJ) and knee joints. Twenty-four adult male New Zealand White rabbits were divided into three groups: a control group as well as TMJ arthritis and knee joint arthritis groups. After sensitization, unilateral arthritis was induced by intra-articular injection with ovalbumin and the contralateral joint was injected with saline 3 weeks after induction of arthritis. Joints were then perfused continuously with saline and samples were collected at 10-min intervals over a 50-min period. The IL-1β concentrations in the samples were then analyzed. After killing the animals, the joints were examined histologically. The IL-1β concentrations in the samples from the arthritic TMJs and knee joints were significantly higher than in the saline-injected and the control joints. Histological signs of chronic arthritis of similar severity were found in both joints. The IL-1β levels in the samples from the arthritic TM and knee joints correlated with the histological severity of the arthritis, including pannus formation. In conclusion, this study shows that IL-1β is released in the synovium of rabbit TMJs and knee joints during antigen-induced arthritis, and that high IL-1β levels in synovial fluid are associated with histological signs of inflammation including, pannus tissue formation.
| Original language | English |
|---|---|
| Pages (from-to) | 539-544 |
| Number of pages | 6 |
| Journal | Archives of Oral Biology |
| Volume | 46 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2001 |
| Externally published | Yes |
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Keywords
- Arthritis
- Interleukin-1β
- Knee joint
- Rabbit
- Temporomandibular joint
ASJC Scopus subject areas
- Dentistry(all)
Cite this
Interleukin-1β in antigen-induced arthritis of the rabbit temporomandibular joint. / Tominaga, Kazuhiro; Alstergren, Per; Kurita, Hiroshi; Matsukawa, Akihiro; Fukuda, Jinichi; Kopp, Sigvard.
In: Archives of Oral Biology, Vol. 46, No. 6, 06.2001, p. 539-544.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interleukin-1β in antigen-induced arthritis of the rabbit temporomandibular joint
AU - Tominaga, Kazuhiro
AU - Alstergren, Per
AU - Kurita, Hiroshi
AU - Matsukawa, Akihiro
AU - Fukuda, Jinichi
AU - Kopp, Sigvard
PY - 2001/6
Y1 - 2001/6
N2 - The aim was to investigate joint perfusate levels of interleukin-1β (1L-1β) in antigen-induced monoarthritis of the rabbit temporomandibular (TMJ) and knee joints. Twenty-four adult male New Zealand White rabbits were divided into three groups: a control group as well as TMJ arthritis and knee joint arthritis groups. After sensitization, unilateral arthritis was induced by intra-articular injection with ovalbumin and the contralateral joint was injected with saline 3 weeks after induction of arthritis. Joints were then perfused continuously with saline and samples were collected at 10-min intervals over a 50-min period. The IL-1β concentrations in the samples were then analyzed. After killing the animals, the joints were examined histologically. The IL-1β concentrations in the samples from the arthritic TMJs and knee joints were significantly higher than in the saline-injected and the control joints. Histological signs of chronic arthritis of similar severity were found in both joints. The IL-1β levels in the samples from the arthritic TM and knee joints correlated with the histological severity of the arthritis, including pannus formation. In conclusion, this study shows that IL-1β is released in the synovium of rabbit TMJs and knee joints during antigen-induced arthritis, and that high IL-1β levels in synovial fluid are associated with histological signs of inflammation including, pannus tissue formation.
AB - The aim was to investigate joint perfusate levels of interleukin-1β (1L-1β) in antigen-induced monoarthritis of the rabbit temporomandibular (TMJ) and knee joints. Twenty-four adult male New Zealand White rabbits were divided into three groups: a control group as well as TMJ arthritis and knee joint arthritis groups. After sensitization, unilateral arthritis was induced by intra-articular injection with ovalbumin and the contralateral joint was injected with saline 3 weeks after induction of arthritis. Joints were then perfused continuously with saline and samples were collected at 10-min intervals over a 50-min period. The IL-1β concentrations in the samples were then analyzed. After killing the animals, the joints were examined histologically. The IL-1β concentrations in the samples from the arthritic TMJs and knee joints were significantly higher than in the saline-injected and the control joints. Histological signs of chronic arthritis of similar severity were found in both joints. The IL-1β levels in the samples from the arthritic TM and knee joints correlated with the histological severity of the arthritis, including pannus formation. In conclusion, this study shows that IL-1β is released in the synovium of rabbit TMJs and knee joints during antigen-induced arthritis, and that high IL-1β levels in synovial fluid are associated with histological signs of inflammation including, pannus tissue formation.
KW - Arthritis
KW - Interleukin-1β
KW - Knee joint
KW - Rabbit
KW - Temporomandibular joint
UR - http://www.scopus.com/inward/record.url?scp=0034744025&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034744025&partnerID=8YFLogxK
U2 - 10.1016/S0003-9969(01)00009-7
DO - 10.1016/S0003-9969(01)00009-7
M3 - Article
C2 - 11311201
AN - SCOPUS:0034744025
VL - 46
SP - 539
EP - 544
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
IS - 6
ER -