Abstract
The aim was to investigate joint perfusate levels of interleukin-1β (1L-1β) in antigen-induced monoarthritis of the rabbit temporomandibular (TMJ) and knee joints. Twenty-four adult male New Zealand White rabbits were divided into three groups: a control group as well as TMJ arthritis and knee joint arthritis groups. After sensitization, unilateral arthritis was induced by intra-articular injection with ovalbumin and the contralateral joint was injected with saline 3 weeks after induction of arthritis. Joints were then perfused continuously with saline and samples were collected at 10-min intervals over a 50-min period. The IL-1β concentrations in the samples were then analyzed. After killing the animals, the joints were examined histologically. The IL-1β concentrations in the samples from the arthritic TMJs and knee joints were significantly higher than in the saline-injected and the control joints. Histological signs of chronic arthritis of similar severity were found in both joints. The IL-1β levels in the samples from the arthritic TM and knee joints correlated with the histological severity of the arthritis, including pannus formation. In conclusion, this study shows that IL-1β is released in the synovium of rabbit TMJs and knee joints during antigen-induced arthritis, and that high IL-1β levels in synovial fluid are associated with histological signs of inflammation including, pannus tissue formation.
Original language | English |
---|---|
Pages (from-to) | 539-544 |
Number of pages | 6 |
Journal | Archives of Oral Biology |
Volume | 46 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jun 2001 |
Externally published | Yes |
Keywords
- Arthritis
- Interleukin-1β
- Knee joint
- Rabbit
- Temporomandibular joint
ASJC Scopus subject areas
- Otorhinolaryngology
- Dentistry(all)
- Cell Biology