Abstract
We have sought to identify transcriptional pathways in adipogenesis using an integrated experimental and computational approach. Here, we employ high-throughput DNase hypersensitivity analysis to find regions of altered chromatin structure surrounding key adipocyte genes. Regions that display differentiation-dependent changes in hypersensitivity were used to predict binding sites for proteins involved in adipogenesis. A high-scoring example was a binding motif for interferon regulatory factor (IRF) family members. Expression of all nine mammalian IRF mRNAs is regulated during adipogenesis, and several bind to the identified motifs in a differentiation-dependent manner. Furthermore, several IRF proteins repress differentiation. This analysis suggests an important role for IRF proteins in adipocyte biology and demonstrates the utility of this approach in identifying cis- and trans-acting factors not previously suspected to participate in adipogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 86-94 |
| Number of pages | 9 |
| Journal | Cell Metabolism |
| Volume | 7 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 9 2008 |
| Externally published | Yes |
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Keywords
- HUMDISEASE
- PROTEINS
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Physiology
Cite this
Interferon Regulatory Factors Are Transcriptional Regulators of Adipogenesis. / Eguchi, Jun; Yan, Qing Wu; Schones, Dustin E.; Kamal, Michael; Hsu, Chung Hsin; Zhang, Michael Q.; Crawford, Gregory E.; Rosen, Evan D.
In: Cell Metabolism, Vol. 7, No. 1, 09.01.2008, p. 86-94.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interferon Regulatory Factors Are Transcriptional Regulators of Adipogenesis
AU - Eguchi, Jun
AU - Yan, Qing Wu
AU - Schones, Dustin E.
AU - Kamal, Michael
AU - Hsu, Chung Hsin
AU - Zhang, Michael Q.
AU - Crawford, Gregory E.
AU - Rosen, Evan D.
PY - 2008/1/9
Y1 - 2008/1/9
N2 - We have sought to identify transcriptional pathways in adipogenesis using an integrated experimental and computational approach. Here, we employ high-throughput DNase hypersensitivity analysis to find regions of altered chromatin structure surrounding key adipocyte genes. Regions that display differentiation-dependent changes in hypersensitivity were used to predict binding sites for proteins involved in adipogenesis. A high-scoring example was a binding motif for interferon regulatory factor (IRF) family members. Expression of all nine mammalian IRF mRNAs is regulated during adipogenesis, and several bind to the identified motifs in a differentiation-dependent manner. Furthermore, several IRF proteins repress differentiation. This analysis suggests an important role for IRF proteins in adipocyte biology and demonstrates the utility of this approach in identifying cis- and trans-acting factors not previously suspected to participate in adipogenesis.
AB - We have sought to identify transcriptional pathways in adipogenesis using an integrated experimental and computational approach. Here, we employ high-throughput DNase hypersensitivity analysis to find regions of altered chromatin structure surrounding key adipocyte genes. Regions that display differentiation-dependent changes in hypersensitivity were used to predict binding sites for proteins involved in adipogenesis. A high-scoring example was a binding motif for interferon regulatory factor (IRF) family members. Expression of all nine mammalian IRF mRNAs is regulated during adipogenesis, and several bind to the identified motifs in a differentiation-dependent manner. Furthermore, several IRF proteins repress differentiation. This analysis suggests an important role for IRF proteins in adipocyte biology and demonstrates the utility of this approach in identifying cis- and trans-acting factors not previously suspected to participate in adipogenesis.
KW - HUMDISEASE
KW - PROTEINS
UR - http://www.scopus.com/inward/record.url?scp=37449021303&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=37449021303&partnerID=8YFLogxK
U2 - 10.1016/j.cmet.2007.11.002
DO - 10.1016/j.cmet.2007.11.002
M3 - Article
C2 - 18177728
AN - SCOPUS:37449021303
VL - 7
SP - 86
EP - 94
JO - Cell Metabolism
JF - Cell Metabolism
SN - 1550-4131
IS - 1
ER -