Abstract
We have sought to identify transcriptional pathways in adipogenesis using an integrated experimental and computational approach. Here, we employ high-throughput DNase hypersensitivity analysis to find regions of altered chromatin structure surrounding key adipocyte genes. Regions that display differentiation-dependent changes in hypersensitivity were used to predict binding sites for proteins involved in adipogenesis. A high-scoring example was a binding motif for interferon regulatory factor (IRF) family members. Expression of all nine mammalian IRF mRNAs is regulated during adipogenesis, and several bind to the identified motifs in a differentiation-dependent manner. Furthermore, several IRF proteins repress differentiation. This analysis suggests an important role for IRF proteins in adipocyte biology and demonstrates the utility of this approach in identifying cis- and trans-acting factors not previously suspected to participate in adipogenesis.
Original language | English |
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Pages (from-to) | 86-94 |
Number of pages | 9 |
Journal | Cell Metabolism |
Volume | 7 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 9 2008 |
Externally published | Yes |
Keywords
- HUMDISEASE
- PROTEINS
ASJC Scopus subject areas
- Physiology
- Molecular Biology
- Cell Biology