Interferon-τ Blocks the Stimulatory Effect of Tumor Necrosis Factor-α on Prostaglandin F2α Synthesis by Bovine Endometrial Stromal Cells

Kiyoshi Okuda, Yuko Kasahara, Shuko Murakami, Hitomi Takahashi, Izabela Woclawek-Potocka, Dariusz J. Skarzynski

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Tumor necrosis factor-α (TNFα) has been shown to be a potent stimulator of prostaglandin (PG) F synthesis in bovine endometrial stromal cells. The aims of the present study were to determine the effect of interferon-τ (IFNτ) on TNFα-stimulated PGF synthesis and the intracellular mechanisms of TNFα and IFNτ action in the stromal cells. When cultured bovine stromal cells were exposed to TNFα (0.006-0.6 nM) for 24 h, the production of PGF and cyclooxygenase (COX)-2 gene expression were stimulated by TNFα (0.06-0.6 nM, P <0.05). Moreover, a specific COX-2 inhibitor (NS-398; 5 nM) blocked the stimulatory effect of TNFα on PGF production (P <0.05). Although IFNτ (0.03-30 ng/ml) did not stimulate basal PGF production in the stromal cells, it suppressed TNFα action in PGF production dose dependently (P <0.05). Moreover, the stimulatory effect of TNFα (0.6 nM) on COX-2 gene expression was completely blocked by IFNτ (30 ng/ml; P <0.05), although the gene expression of COX-2 was not influenced by IFNτ. The overall results indicate that the stimulatory effect of TNFα on PGF. production is mediated by the up-regulation of COX-2 gene expression and suggest that one of the mechanisms of the inhibitory effect of IFNτ on luteolysis is the inhibition of TNFα action in PGF production in the stromal cells by the down-regulation of COX-2 gene expression stimulated by TNFα.

Original languageEnglish
Pages (from-to)191-197
Number of pages7
JournalBiology of Reproduction
Volume70
Issue number1
DOIs
Publication statusPublished - Jan 2004

Fingerprint

Dinoprost
Stromal Cells
Interferons
Tumor Necrosis Factor-alpha
Cyclooxygenase 2
Gene Expression
Prostaglandins F
Luteolysis
Cyclooxygenase 2 Inhibitors
Up-Regulation
Down-Regulation

Keywords

  • Cytokines
  • Female reproductive tract
  • Mechanisms of hormone action
  • Ovulatory cycle
  • Uterus

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Interferon-τ Blocks the Stimulatory Effect of Tumor Necrosis Factor-α on Prostaglandin F2α Synthesis by Bovine Endometrial Stromal Cells. / Okuda, Kiyoshi; Kasahara, Yuko; Murakami, Shuko; Takahashi, Hitomi; Woclawek-Potocka, Izabela; Skarzynski, Dariusz J.

In: Biology of Reproduction, Vol. 70, No. 1, 01.2004, p. 191-197.

Research output: Contribution to journalArticle

Okuda, Kiyoshi ; Kasahara, Yuko ; Murakami, Shuko ; Takahashi, Hitomi ; Woclawek-Potocka, Izabela ; Skarzynski, Dariusz J. / Interferon-τ Blocks the Stimulatory Effect of Tumor Necrosis Factor-α on Prostaglandin F2α Synthesis by Bovine Endometrial Stromal Cells. In: Biology of Reproduction. 2004 ; Vol. 70, No. 1. pp. 191-197.
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abstract = "Tumor necrosis factor-α (TNFα) has been shown to be a potent stimulator of prostaglandin (PG) F2α synthesis in bovine endometrial stromal cells. The aims of the present study were to determine the effect of interferon-τ (IFNτ) on TNFα-stimulated PGF 2α synthesis and the intracellular mechanisms of TNFα and IFNτ action in the stromal cells. When cultured bovine stromal cells were exposed to TNFα (0.006-0.6 nM) for 24 h, the production of PGF 2α and cyclooxygenase (COX)-2 gene expression were stimulated by TNFα (0.06-0.6 nM, P <0.05). Moreover, a specific COX-2 inhibitor (NS-398; 5 nM) blocked the stimulatory effect of TNFα on PGF 2α production (P <0.05). Although IFNτ (0.03-30 ng/ml) did not stimulate basal PGF2α production in the stromal cells, it suppressed TNFα action in PGF2α production dose dependently (P <0.05). Moreover, the stimulatory effect of TNFα (0.6 nM) on COX-2 gene expression was completely blocked by IFNτ (30 ng/ml; P <0.05), although the gene expression of COX-2 was not influenced by IFNτ. The overall results indicate that the stimulatory effect of TNFα on PGF2α. production is mediated by the up-regulation of COX-2 gene expression and suggest that one of the mechanisms of the inhibitory effect of IFNτ on luteolysis is the inhibition of TNFα action in PGF2α production in the stromal cells by the down-regulation of COX-2 gene expression stimulated by TNFα.",
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AB - Tumor necrosis factor-α (TNFα) has been shown to be a potent stimulator of prostaglandin (PG) F2α synthesis in bovine endometrial stromal cells. The aims of the present study were to determine the effect of interferon-τ (IFNτ) on TNFα-stimulated PGF 2α synthesis and the intracellular mechanisms of TNFα and IFNτ action in the stromal cells. When cultured bovine stromal cells were exposed to TNFα (0.006-0.6 nM) for 24 h, the production of PGF 2α and cyclooxygenase (COX)-2 gene expression were stimulated by TNFα (0.06-0.6 nM, P <0.05). Moreover, a specific COX-2 inhibitor (NS-398; 5 nM) blocked the stimulatory effect of TNFα on PGF 2α production (P <0.05). Although IFNτ (0.03-30 ng/ml) did not stimulate basal PGF2α production in the stromal cells, it suppressed TNFα action in PGF2α production dose dependently (P <0.05). Moreover, the stimulatory effect of TNFα (0.6 nM) on COX-2 gene expression was completely blocked by IFNτ (30 ng/ml; P <0.05), although the gene expression of COX-2 was not influenced by IFNτ. The overall results indicate that the stimulatory effect of TNFα on PGF2α. production is mediated by the up-regulation of COX-2 gene expression and suggest that one of the mechanisms of the inhibitory effect of IFNτ on luteolysis is the inhibition of TNFα action in PGF2α production in the stromal cells by the down-regulation of COX-2 gene expression stimulated by TNFα.

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