Abstract
Tumor necrosis factor-α (TNFα) has been shown to be a potent stimulator of prostaglandin (PG) F2α synthesis in bovine endometrial stromal cells. The aims of the present study were to determine the effect of interferon-τ (IFNτ) on TNFα-stimulated PGF 2α synthesis and the intracellular mechanisms of TNFα and IFNτ action in the stromal cells. When cultured bovine stromal cells were exposed to TNFα (0.006-0.6 nM) for 24 h, the production of PGF 2α and cyclooxygenase (COX)-2 gene expression were stimulated by TNFα (0.06-0.6 nM, P <0.05). Moreover, a specific COX-2 inhibitor (NS-398; 5 nM) blocked the stimulatory effect of TNFα on PGF 2α production (P <0.05). Although IFNτ (0.03-30 ng/ml) did not stimulate basal PGF2α production in the stromal cells, it suppressed TNFα action in PGF2α production dose dependently (P <0.05). Moreover, the stimulatory effect of TNFα (0.6 nM) on COX-2 gene expression was completely blocked by IFNτ (30 ng/ml; P <0.05), although the gene expression of COX-2 was not influenced by IFNτ. The overall results indicate that the stimulatory effect of TNFα on PGF2α. production is mediated by the up-regulation of COX-2 gene expression and suggest that one of the mechanisms of the inhibitory effect of IFNτ on luteolysis is the inhibition of TNFα action in PGF2α production in the stromal cells by the down-regulation of COX-2 gene expression stimulated by TNFα.
| Original language | English |
|---|---|
| Pages (from-to) | 191-197 |
| Number of pages | 7 |
| Journal | Biology of Reproduction |
| Volume | 70 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2004 |
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Keywords
- Cytokines
- Female reproductive tract
- Mechanisms of hormone action
- Ovulatory cycle
- Uterus
ASJC Scopus subject areas
- Cell Biology
- Developmental Biology
- Embryology
Cite this
Interferon-τ Blocks the Stimulatory Effect of Tumor Necrosis Factor-α on Prostaglandin F2α Synthesis by Bovine Endometrial Stromal Cells. / Okuda, Kiyoshi; Kasahara, Yuko; Murakami, Shuko; Takahashi, Hitomi; Woclawek-Potocka, Izabela; Skarzynski, Dariusz J.
In: Biology of Reproduction, Vol. 70, No. 1, 01.2004, p. 191-197.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interferon-τ Blocks the Stimulatory Effect of Tumor Necrosis Factor-α on Prostaglandin F2α Synthesis by Bovine Endometrial Stromal Cells
AU - Okuda, Kiyoshi
AU - Kasahara, Yuko
AU - Murakami, Shuko
AU - Takahashi, Hitomi
AU - Woclawek-Potocka, Izabela
AU - Skarzynski, Dariusz J.
PY - 2004/1
Y1 - 2004/1
N2 - Tumor necrosis factor-α (TNFα) has been shown to be a potent stimulator of prostaglandin (PG) F2α synthesis in bovine endometrial stromal cells. The aims of the present study were to determine the effect of interferon-τ (IFNτ) on TNFα-stimulated PGF 2α synthesis and the intracellular mechanisms of TNFα and IFNτ action in the stromal cells. When cultured bovine stromal cells were exposed to TNFα (0.006-0.6 nM) for 24 h, the production of PGF 2α and cyclooxygenase (COX)-2 gene expression were stimulated by TNFα (0.06-0.6 nM, P <0.05). Moreover, a specific COX-2 inhibitor (NS-398; 5 nM) blocked the stimulatory effect of TNFα on PGF 2α production (P <0.05). Although IFNτ (0.03-30 ng/ml) did not stimulate basal PGF2α production in the stromal cells, it suppressed TNFα action in PGF2α production dose dependently (P <0.05). Moreover, the stimulatory effect of TNFα (0.6 nM) on COX-2 gene expression was completely blocked by IFNτ (30 ng/ml; P <0.05), although the gene expression of COX-2 was not influenced by IFNτ. The overall results indicate that the stimulatory effect of TNFα on PGF2α. production is mediated by the up-regulation of COX-2 gene expression and suggest that one of the mechanisms of the inhibitory effect of IFNτ on luteolysis is the inhibition of TNFα action in PGF2α production in the stromal cells by the down-regulation of COX-2 gene expression stimulated by TNFα.
AB - Tumor necrosis factor-α (TNFα) has been shown to be a potent stimulator of prostaglandin (PG) F2α synthesis in bovine endometrial stromal cells. The aims of the present study were to determine the effect of interferon-τ (IFNτ) on TNFα-stimulated PGF 2α synthesis and the intracellular mechanisms of TNFα and IFNτ action in the stromal cells. When cultured bovine stromal cells were exposed to TNFα (0.006-0.6 nM) for 24 h, the production of PGF 2α and cyclooxygenase (COX)-2 gene expression were stimulated by TNFα (0.06-0.6 nM, P <0.05). Moreover, a specific COX-2 inhibitor (NS-398; 5 nM) blocked the stimulatory effect of TNFα on PGF 2α production (P <0.05). Although IFNτ (0.03-30 ng/ml) did not stimulate basal PGF2α production in the stromal cells, it suppressed TNFα action in PGF2α production dose dependently (P <0.05). Moreover, the stimulatory effect of TNFα (0.6 nM) on COX-2 gene expression was completely blocked by IFNτ (30 ng/ml; P <0.05), although the gene expression of COX-2 was not influenced by IFNτ. The overall results indicate that the stimulatory effect of TNFα on PGF2α. production is mediated by the up-regulation of COX-2 gene expression and suggest that one of the mechanisms of the inhibitory effect of IFNτ on luteolysis is the inhibition of TNFα action in PGF2α production in the stromal cells by the down-regulation of COX-2 gene expression stimulated by TNFα.
KW - Cytokines
KW - Female reproductive tract
KW - Mechanisms of hormone action
KW - Ovulatory cycle
KW - Uterus
UR - http://www.scopus.com/inward/record.url?scp=0347949744&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347949744&partnerID=8YFLogxK
U2 - 10.1095/biolreprod.103.019083
DO - 10.1095/biolreprod.103.019083
M3 - Article
C2 - 13679315
AN - SCOPUS:0347949744
VL - 70
SP - 191
EP - 197
JO - Biology of Reproduction
JF - Biology of Reproduction
SN - 0006-3363
IS - 1
ER -