Interferon-τ Blocks the Stimulatory Effect of Tumor Necrosis Factor-α on Prostaglandin F Synthesis by Bovine Endometrial Stromal Cells

Kiyoshi Okuda, Yuko Kasahara, Shuko Murakami, Hitomi Takahashi, Izabela Woclawek-Potocka, Dariusz J. Skarzynski

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Tumor necrosis factor-α (TNFα) has been shown to be a potent stimulator of prostaglandin (PG) F synthesis in bovine endometrial stromal cells. The aims of the present study were to determine the effect of interferon-τ (IFNτ) on TNFα-stimulated PGF synthesis and the intracellular mechanisms of TNFα and IFNτ action in the stromal cells. When cultured bovine stromal cells were exposed to TNFα (0.006-0.6 nM) for 24 h, the production of PGF and cyclooxygenase (COX)-2 gene expression were stimulated by TNFα (0.06-0.6 nM, P < 0.05). Moreover, a specific COX-2 inhibitor (NS-398; 5 nM) blocked the stimulatory effect of TNFα on PGF production (P < 0.05). Although IFNτ (0.03-30 ng/ml) did not stimulate basal PGF production in the stromal cells, it suppressed TNFα action in PGF production dose dependently (P < 0.05). Moreover, the stimulatory effect of TNFα (0.6 nM) on COX-2 gene expression was completely blocked by IFNτ (30 ng/ml; P < 0.05), although the gene expression of COX-2 was not influenced by IFNτ. The overall results indicate that the stimulatory effect of TNFα on PGF. production is mediated by the up-regulation of COX-2 gene expression and suggest that one of the mechanisms of the inhibitory effect of IFNτ on luteolysis is the inhibition of TNFα action in PGF production in the stromal cells by the down-regulation of COX-2 gene expression stimulated by TNFα.

Original languageEnglish
Pages (from-to)191-197
Number of pages7
JournalBiology of reproduction
Volume70
Issue number1
DOIs
Publication statusPublished - Jan 1 2004

Keywords

  • Cytokines
  • Female reproductive tract
  • Mechanisms of hormone action
  • Ovulatory cycle
  • Uterus

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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