Abstract
Epidermal keratinocytes contain 15-lipoxygenase, which generates 15-hydroxyeicosatetraenoic acid, a major metabolite of arachidonic acid. Although two isozymes, 15-lipoxygenase-1 and -2, exist, it remains unclear which isozyme plays an important role in inflammatory processes and proliferative skin diseases. In the present study, we demonstrated that 15-lipoxygenase-2 expression was increased in normal human epidermal keratinocytes and HaCaT cells treated with interferon-γ (200 U/ml), while no induction of 15-lipoxygenase-1 was observed. Under the same culture conditions, no 15-lipoxygenase-2 was expressed by a carcinoma cell line, A431. Weak expression of 15-lipoxygenase-2 was observed in the basal cell layer of non-lesional psoriatic skin by in situ hybridization and immunostaining, whereas strong expression of 15-lipoxygenase-2 was observed in all living layers of psoriatic lesions. Actinic keratosis and squamous cell carcinomas showed a variable immunostaining pattern for 15-lipoxygenase-2. These results indicate that 15-lipoxygenase-2 is implicated in interferon-γ-induced inflammatory processes in normal human epidermal keratinocytes and psoriatic skin.
| Original language | English |
|---|---|
| Pages (from-to) | 141-145 |
| Number of pages | 5 |
| Journal | European Journal of Dermatology |
| Volume | 16 |
| Issue number | 2 |
| Publication status | Published - Mar 2006 |
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Keywords
- 15-lipoxygenase-2
- Interferon-γ
- Keratinocytes
- Psoriasis
ASJC Scopus subject areas
- Dermatology
Cite this
Interferon-γ-induced 15-lipoxygenase-2 expression in normal human epidermal keratinocytes and a pathogenic link to psoriasis vulgaris. / Setsu, Namiko; Matsuura, Hironori; Hirakawa, Satoshi; Arata, Jirô; Iwatsuki, Keiji.
In: European Journal of Dermatology, Vol. 16, No. 2, 03.2006, p. 141-145.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interferon-γ-induced 15-lipoxygenase-2 expression in normal human epidermal keratinocytes and a pathogenic link to psoriasis vulgaris
AU - Setsu, Namiko
AU - Matsuura, Hironori
AU - Hirakawa, Satoshi
AU - Arata, Jirô
AU - Iwatsuki, Keiji
PY - 2006/3
Y1 - 2006/3
N2 - Epidermal keratinocytes contain 15-lipoxygenase, which generates 15-hydroxyeicosatetraenoic acid, a major metabolite of arachidonic acid. Although two isozymes, 15-lipoxygenase-1 and -2, exist, it remains unclear which isozyme plays an important role in inflammatory processes and proliferative skin diseases. In the present study, we demonstrated that 15-lipoxygenase-2 expression was increased in normal human epidermal keratinocytes and HaCaT cells treated with interferon-γ (200 U/ml), while no induction of 15-lipoxygenase-1 was observed. Under the same culture conditions, no 15-lipoxygenase-2 was expressed by a carcinoma cell line, A431. Weak expression of 15-lipoxygenase-2 was observed in the basal cell layer of non-lesional psoriatic skin by in situ hybridization and immunostaining, whereas strong expression of 15-lipoxygenase-2 was observed in all living layers of psoriatic lesions. Actinic keratosis and squamous cell carcinomas showed a variable immunostaining pattern for 15-lipoxygenase-2. These results indicate that 15-lipoxygenase-2 is implicated in interferon-γ-induced inflammatory processes in normal human epidermal keratinocytes and psoriatic skin.
AB - Epidermal keratinocytes contain 15-lipoxygenase, which generates 15-hydroxyeicosatetraenoic acid, a major metabolite of arachidonic acid. Although two isozymes, 15-lipoxygenase-1 and -2, exist, it remains unclear which isozyme plays an important role in inflammatory processes and proliferative skin diseases. In the present study, we demonstrated that 15-lipoxygenase-2 expression was increased in normal human epidermal keratinocytes and HaCaT cells treated with interferon-γ (200 U/ml), while no induction of 15-lipoxygenase-1 was observed. Under the same culture conditions, no 15-lipoxygenase-2 was expressed by a carcinoma cell line, A431. Weak expression of 15-lipoxygenase-2 was observed in the basal cell layer of non-lesional psoriatic skin by in situ hybridization and immunostaining, whereas strong expression of 15-lipoxygenase-2 was observed in all living layers of psoriatic lesions. Actinic keratosis and squamous cell carcinomas showed a variable immunostaining pattern for 15-lipoxygenase-2. These results indicate that 15-lipoxygenase-2 is implicated in interferon-γ-induced inflammatory processes in normal human epidermal keratinocytes and psoriatic skin.
KW - 15-lipoxygenase-2
KW - Interferon-γ
KW - Keratinocytes
KW - Psoriasis
UR - http://www.scopus.com/inward/record.url?scp=33645571194&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645571194&partnerID=8YFLogxK
M3 - Article
C2 - 16581564
AN - SCOPUS:33645571194
VL - 16
SP - 141
EP - 145
JO - European Journal of Dermatology
JF - European Journal of Dermatology
SN - 1167-1122
IS - 2
ER -