Intercellular and intracellular cilia orientation is coordinated by CELSR1 and CAMSAP3 in oviduct multi-ciliated cells

Fumiko Matsukawa Usami, Masaki Arata, Dongbo Shi, Sanae Oka, Yoko Higuchi, Fadel Tissir, Masatoshi Takeichi, Toshihiko Fujimori

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The molecular mechanisms by which cilia orientation is coordinated within and between multi-ciliated cells (MCCs) are not fully understood. In the mouse oviduct, MCCs exhibit a characteristic basal body (BB) orientation and microtubule gradient along the tissue axis. The intracellular polarities were moderately maintained in cells lacking CELSR1 (cadherin EGF LAG seven-pass G-type receptor 1), a planar cell polarity (PCP) factor involved in tissue polarity regulation, although the intercellular coordination of the polarities was disrupted. However, CAMSAP3 (calmodulin-regulated spectrin-associated protein 3), a microtubule minus-end regulator, was found to be critical for determining the intracellular BB orientation. CAMSAP3 localized to the base of cilia in a polarized manner, and its mutation led to the disruption of intracellular coordination of BB orientation, as well as the assembly of microtubules interconnecting BBs, without affecting PCP factor localization. Thus, bothCELSR1 and CAMSAP3 are responsible for BB orientation but in distinct ways; their cooperation should therefore be critical for generating functional multi-ciliated tissues.

Original languageEnglish
Article numberjcs257006
JournalJournal of cell science
Volume134
Issue number4
DOIs
Publication statusPublished - Feb 2021
Externally publishedYes

Keywords

  • Basal body
  • Cytoskeleton
  • Oviduct
  • Planar cell polarity
  • Reproduction
  • Super-resolution microscopy

ASJC Scopus subject areas

  • Cell Biology

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