Interaction of the halobacterial transducer to a halorhodopsin mutant engineered so as to bind the transducer: Cl- circulation within the extracellular channel

Chisa Hasegawa, Takashi Kikukawa, Seiji Miyauchi, Akiteru Seki, Yuki Sudo, Megumi Kubo, Makoto Demura, Naoki Kamo

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

An alkali-halophilic archaeum, Natronomonas pharaonis, contains two rhodopsins that are halorhodopsin (phR), a light-driven inward Cl- pump and phoborhodopsin (ppR), the receptor of negative phototaxis functioning by forming a signaling complex with a transducer, pHtrII (Sudo Y. et al., J. Mol. Biol. 357 [2006] 1274). Previously, we reported that the phR double mutant, P240T/F250YphR, can bind with pHtrII. This mutant itself can transport Cl-, while the net transport was stopped upon formation of the complex. The flash-photolysis data were analyzed by a scheme in which phR→P1→P2→P3→P 4→phR. The P3 of the wild-type and the double mutant contained two components, X- and O-intermediates. After the complex formation, however, the P3 of the double mutant lacked the X-intermediate. These observations imply that the X-intermediate (probably the N-intermediate) is the state having Cl- in the cytoplasmic binding site and that the complex undergoes an extracellular Cl- circulation because of the inhibition of formation of the X-intermediate.

Original languageEnglish
Pages (from-to)293-302
Number of pages10
JournalPhotochemistry and Photobiology
Volume83
Issue number2
DOIs
Publication statusPublished - Mar 2007
Externally publishedYes

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Halorhodopsins
Rhodopsin
Photolysis
Archaea
Alkalies
Transducers
transducers
Binding Sites
Pumps
Light
interactions
flash
photolysis
alkalies
pumps
Phototaxis
Inhibition (Psychology)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Interaction of the halobacterial transducer to a halorhodopsin mutant engineered so as to bind the transducer : Cl- circulation within the extracellular channel. / Hasegawa, Chisa; Kikukawa, Takashi; Miyauchi, Seiji; Seki, Akiteru; Sudo, Yuki; Kubo, Megumi; Demura, Makoto; Kamo, Naoki.

In: Photochemistry and Photobiology, Vol. 83, No. 2, 03.2007, p. 293-302.

Research output: Contribution to journalArticle

Hasegawa, Chisa ; Kikukawa, Takashi ; Miyauchi, Seiji ; Seki, Akiteru ; Sudo, Yuki ; Kubo, Megumi ; Demura, Makoto ; Kamo, Naoki. / Interaction of the halobacterial transducer to a halorhodopsin mutant engineered so as to bind the transducer : Cl- circulation within the extracellular channel. In: Photochemistry and Photobiology. 2007 ; Vol. 83, No. 2. pp. 293-302.
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