Interaction of SNARE complexes with P/Q-type calcium channels in rat cerebellar synaptosomes

Nicole Martin-Moutot, Nathalie Charvin, Christian Leveque, Kazuki Sato, Tei-ichi Nishiki, Shunji Kozaki, Masami Takahashi, Michael Seagar

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

P- and Q-type calcium channels, which trigger rapid neurotransmitter release at many mammalian synapses, are blocked by ω-conotoxin MVIIC. 125I-ω-Conotoxin MVIIC binding to rat cerebellar synaptosomes was not displaced by ω-conotoxins GVIA or MVIIA (K(i) > 1 μM), which are selective for N-type calcium channels. Solubilized 125I-ω-conotoxin MVIIC receptors were specifically recognized by antibodies directed against α1A calcium channel subunits, proteins known to constitute a pore with P/Q-like channel properties. Antibodies against syntaxin 1, SNAP 25, and VAMP 2 (synaptobrevin) each immunoprecipitated a similar fraction (20-40%) of ω- conotoxin MVIIC receptors. Immunoprecipitation was not additive, suggesting that heterotrimeric (SNARE) complexes containing these three proteins interact with P/Q-type calcium channels. Immobilized monoclonal anti-syntaxin antibodies retained α1A calcium channel subunits of 220, 180 and 160 kDa monitored by immunoblotting with site directed antibodies. Synaptotagmin was detected in channel-associated complexes, but not synaptophysin, Rab 3A nor rat cysteine string protein. Trimeric SNARE complexes are implicated in calcium-dependent exocytosis, a process thought to be regulated by synaptotagmin. Our results indicate that these proteins interact with P/Q- type calcium channels, which may optimize their location within domains of calcium influx.

Original languageEnglish
Pages (from-to)6567-6570
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number12
DOIs
Publication statusPublished - Mar 22 1996
Externally publishedYes

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Q-Type Calcium Channels
P-Type Calcium Channels
Conotoxins
SNARE Proteins
Synaptosomes
Rats
Synaptotagmins
Antibodies
Calcium Channels
Vesicle-Associated Membrane Protein 2
Syntaxin 1
N-Type Calcium Channels
R-SNARE Proteins
Qa-SNARE Proteins
Calcium
Synaptophysin
Exocytosis
Protein Subunits
Immunoprecipitation
Immunoblotting

ASJC Scopus subject areas

  • Biochemistry

Cite this

Martin-Moutot, N., Charvin, N., Leveque, C., Sato, K., Nishiki, T., Kozaki, S., ... Seagar, M. (1996). Interaction of SNARE complexes with P/Q-type calcium channels in rat cerebellar synaptosomes. Journal of Biological Chemistry, 271(12), 6567-6570. https://doi.org/10.1074/jbc.271.12.6567

Interaction of SNARE complexes with P/Q-type calcium channels in rat cerebellar synaptosomes. / Martin-Moutot, Nicole; Charvin, Nathalie; Leveque, Christian; Sato, Kazuki; Nishiki, Tei-ichi; Kozaki, Shunji; Takahashi, Masami; Seagar, Michael.

In: Journal of Biological Chemistry, Vol. 271, No. 12, 22.03.1996, p. 6567-6570.

Research output: Contribution to journalArticle

Martin-Moutot, N, Charvin, N, Leveque, C, Sato, K, Nishiki, T, Kozaki, S, Takahashi, M & Seagar, M 1996, 'Interaction of SNARE complexes with P/Q-type calcium channels in rat cerebellar synaptosomes', Journal of Biological Chemistry, vol. 271, no. 12, pp. 6567-6570. https://doi.org/10.1074/jbc.271.12.6567
Martin-Moutot N, Charvin N, Leveque C, Sato K, Nishiki T, Kozaki S et al. Interaction of SNARE complexes with P/Q-type calcium channels in rat cerebellar synaptosomes. Journal of Biological Chemistry. 1996 Mar 22;271(12):6567-6570. https://doi.org/10.1074/jbc.271.12.6567
Martin-Moutot, Nicole ; Charvin, Nathalie ; Leveque, Christian ; Sato, Kazuki ; Nishiki, Tei-ichi ; Kozaki, Shunji ; Takahashi, Masami ; Seagar, Michael. / Interaction of SNARE complexes with P/Q-type calcium channels in rat cerebellar synaptosomes. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 12. pp. 6567-6570.
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