Interaction of polystyrene microspheres with liver cells: Roles of membrane receptors and serum proteins

Ken Ichi Ogawara, Minoru Yoshida, Yoshinobu Takakura, Mitsuru Hashida, Kazutaka Higaki, Toshikiro Kimura

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Our previous studies have demonstrated that serum would play an important role in the hepatic disposition of polystyrene microspheres (MS) and that complement C3 should be involved as the serum opsonin. In this study, we tried to identify the entity of other serum opsonins and dysopsonin for the hepatic uptake of MSs with particle sizes of 50 nm (MS-50) and 500 nm (MS-500) by isolated liver perfusion studies using a recirculation procedure in rats. Pretreatment of the liver by trypsin significantly suppressed the serum-dependent hepatic uptake of both MSs, suggesting that some protein components on the cell surface should be necessary for the serum-dependent phagocytosis of MSs. Pretreatment of the serum by the anti-fibronectin antibody resulted in a significant reduction in the hepatic disposition of MS-500 (49% of control), suggesting that fibronectin should also work as the opsonin for the hepatic uptake of MS-500. The hepatic disposition of both MSs in the presence of serum was inhibited by the addition of N- acetylgalactosamine into the perfusate, suggesting the possible involvement of lectin in the serum-dependent hepatic uptake of MSs. Furthermore, a more intensive hepatic disposition of MSs was observed in the presence of plasma compared with that in the presence of serum in the perfusate, suggesting the possible involvement of blood coagulation factors, such as fibrinogen, as the opsonin in the hepatic disposition of MSs.

Original languageEnglish
Pages (from-to)165-172
Number of pages8
JournalBiochimica et Biophysica Acta - General Subjects
Volume1472
Issue number1-2
DOIs
Publication statusPublished - Oct 18 1999

Fingerprint

Opsonin Proteins
Polystyrenes
Microspheres
Liver
Blood Proteins
Membrane Proteins
Cell Membrane
Membranes
Serum
Fibronectins
Acetylgalactosamine
Complement C3
Blood Coagulation Factors
Lectins
Trypsin
Fibrinogen
Rats
Particle size
Plasmas
Antibodies

Keywords

  • Dysopsonin
  • Hepatic uptake
  • Opsonin
  • Polystyrene microsphere
  • Receptor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Interaction of polystyrene microspheres with liver cells : Roles of membrane receptors and serum proteins. / Ogawara, Ken Ichi; Yoshida, Minoru; Takakura, Yoshinobu; Hashida, Mitsuru; Higaki, Kazutaka; Kimura, Toshikiro.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1472, No. 1-2, 18.10.1999, p. 165-172.

Research output: Contribution to journalArticle

Ogawara, Ken Ichi ; Yoshida, Minoru ; Takakura, Yoshinobu ; Hashida, Mitsuru ; Higaki, Kazutaka ; Kimura, Toshikiro. / Interaction of polystyrene microspheres with liver cells : Roles of membrane receptors and serum proteins. In: Biochimica et Biophysica Acta - General Subjects. 1999 ; Vol. 1472, No. 1-2. pp. 165-172.
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