Interaction of polyphenols with proteins: Binding of (-)-epigallocatechin gallate to serum albumin, estimated by induced circular dichroism

Akiko Nozaki, Mami Hori, Toshikiro Kimura, Hideyuki Lto, Tsutomu Hatano

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The binding of (-)-epigallocatechin gallate (EGCG), a representative natural polyphenol, to human serum albumin (HSA) and bovine serum albumin (BSA) was investigated using induced circular dichroism (CD). The site of the binding EGCG-HSA was analyzed based on the competition with drugs with known binding sites on HSA, such as phenylbutazone (PB) and diazepam (DP). Double-reciprocal plot analyses showed the competitive relations with the site-I- (PB and tolbutamide, TB) and site-II-binding drugs (DP and ibuprofen, IP) indicating the binding of EGCG to sites I and II on HSA, while digitoxin (DG), a site-III-binding drug, did not affect the binding of EGCG. In an analogous way, the competitive relations were observed between EGCG and the site-I- (PB and TB) and site-II-binding (ethacrynic acid, EA) drugs for the binding of EGCG and BSA. The site-III drug DG also showed competitive binding with EGCG to BSA. The binding of EGCG to the albumins indicated its affinity to sites I and II on HSA, while competitive binding for all three sites was observed on BSA.

Original languageEnglish
Pages (from-to)224-228
Number of pages5
JournalChemical and Pharmaceutical Bulletin
Volume57
Issue number2
DOIs
Publication statusPublished - Feb 2009

Fingerprint

Polyphenols
Circular Dichroism
Protein Binding
Serum Albumin
Bovine Serum Albumin
Phenylbutazone
Binding Sites
Digitoxin
Tolbutamide
Competitive Binding
Pharmaceutical Preparations
Diazepam
Ethacrynic Acid
epigallocatechin gallate
Ibuprofen
Albumins

Keywords

  • (-)-epigallocatechin gallate
  • Binding
  • Circular dichroism
  • Interaction
  • Polyphenol
  • Serum albumin

ASJC Scopus subject areas

  • Drug Discovery
  • Chemistry(all)

Cite this

Interaction of polyphenols with proteins : Binding of (-)-epigallocatechin gallate to serum albumin, estimated by induced circular dichroism. / Nozaki, Akiko; Hori, Mami; Kimura, Toshikiro; Lto, Hideyuki; Hatano, Tsutomu.

In: Chemical and Pharmaceutical Bulletin, Vol. 57, No. 2, 02.2009, p. 224-228.

Research output: Contribution to journalArticle

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