Interaction of pituitary hormones and expression of clock genes modulated by bone morphogenetic protein-4 and melatonin

Naoko Tsukamoto-Yamauchi, Tomohiro Terasaka, Yasumasa Iwasaki, Fumio Otsuka

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Functional interaction of clock genes and pituitary hormones was investigated by focusing on bone morphogenetic protein (BMP)-4 and melatonin actions in anterior pituitary cells. A significant correlation between the mRNA expression of proopiomelanocortin (POMC) and Per2 was revealed in serial cultures of corticotrope AtT20 cells. Knockdown of Per2 expression by siRNA in AtT20 cells resulted in a significant reduction of POMC mRNA level with or without corticotropin-releasing hormone (CRH) stimulation. Treatments with BMP-4 and melatonin, both of which suppress POMC expression, reduced Per2 mRNA as well as protein levels in AtT20 cells. On the other hand, in lactosomatotrope GH3 cells, an expressional correlation was found between prolactin (PRL) and Clock mRNA levels, which was attenuated in the presence of forskolin treatment. The siRNA-mediated knockdown of Clock expression, but not that of Bmal1, significantly reduced PRL mRNA levels in GH3 cells. Interestingly, Clock mRNA and protein levels did not fluctuate with melatonin, BMP-4 or forskolin treatment, although Bmal1 expression was significantly increased by forskolin treatment. Collectively, a significant correlation between the expression of POMC and Per2 and that between PRL and Clock were uncovered in corticotrope and lactosomatotrope cells, respectively. Per2 expression was inhibited by POMC modulators including melatonin and BMP-4, while Clock expression was steadily maintained. Thus, the effects of melatonin and BMP-4 on clock gene expression may imply differential stability of circadian rhythms of adrenocorticotropin (ACTH) and PRL secreted from the anterior pituitary.

Original languageEnglish
Pages (from-to)172-177
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume459
Issue number1
DOIs
Publication statusPublished - Feb 9 2015

Fingerprint

Bone Morphogenetic Protein 4
Pituitary Hormones
Melatonin
Pro-Opiomelanocortin
Clocks
Genes
Gene Expression
Prolactin
Messenger RNA
Colforsin
Adrenocorticotropic Hormone
Small Interfering RNA
Corticotropin-Releasing Hormone
Circadian Rhythm
Gene expression
Modulators
Proteins

Keywords

  • Bone morphogenetic protein (BMP)
  • Melatonin
  • Pituitary cells
  • Prolactin (PRL)
  • Proopiomelanocortin (POMC)

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Interaction of pituitary hormones and expression of clock genes modulated by bone morphogenetic protein-4 and melatonin. / Tsukamoto-Yamauchi, Naoko; Terasaka, Tomohiro; Iwasaki, Yasumasa; Otsuka, Fumio.

In: Biochemical and Biophysical Research Communications, Vol. 459, No. 1, 09.02.2015, p. 172-177.

Research output: Contribution to journalArticle

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