Interaction of immunoglobulin with cytomegalovirus-infected cells

Nobuyasu Aiba, Atsuko Shiraki, Misako Yajima, Yukari Oyama, Yoshihiro Yoshida, Ayumu Ohno, Hiroshi Yamada, Masaya Takemoto, Tohru Daikoku, Kimiyasu Shiraki

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Intravenous immunoglobulin (IVIG) is used to treat or prevent severe viral infection, especially cytomegalovirus (CMV) infections. IVIG was characterized to understand its interaction with CMV-infected cells. IVIG retarded CMV spread and reduced virus yields depending on the neutralizing (NT) antibody titer. Immediate early protein synthesis was reduced by IVIG in 3 to 15 h, and IVIG specifically reduced the ratio of 66/68k protein synthesis among immediate early proteins in an NT antibody-dependent manner between 4 and 8 h after infection, indicating that antigenic modulation of CMV-infected cells by IVIG reduced viral protein synthesis and virus production. The half-life of antibody bound to CMV-infected cells was 3.8 h. NT antibody titers to varicella-zoster virus (VZV) and CMV in IVIG were dose dependently absorbed by cells infected with VZV and CMV, respectively, but the antibody titers to CMV and VZV, respectively, were not affected. NT antibody in 0.3 mL of IVIG (15 mg) was specifically absorbed by 10 8 CMV-infected cells and 10 7 VZV-infected cells, suggesting that the NT antibody in IVIG might be inactivated by one-tenth of a similar volume of CMV-infected or VZV-infected cells. Various antiviral activities of IVIG may contribute to control and alleviation of CMV infection.

Original languageEnglish
Pages (from-to)500-507
Number of pages8
JournalViral Immunology
Volume30
Issue number7
DOIs
Publication statusPublished - Sep 2017
Externally publishedYes

Keywords

  • ADCC
  • antigenic modulation
  • cytomegalovirus
  • intravenous immunoglobulin
  • varicella-zoster virus

ASJC Scopus subject areas

  • Immunology
  • Molecular Medicine
  • Virology

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