Abstract
We reported previously that human herpesvirus 6 (HHV-6) suppresses hematopoietic colony formation of erythroid (BFU-E), granulocyte/macrophage (CFU-GM), and megakaryocyte (CFU-Meg) lineages in vitro. Here we describe the interaction between HHV-6 and human CD34+ cells, which are a major source of hematopoietic progenitor cells. CD34+ cells were immunomagnetically isolated from cord blood mononuclear cells using anti-CD34+ antibodies coated onto either Dynabeads™ or MACS beads. The CD34+ population selected with Dynabeads showed a broad range of fluorescence. The population selected with MACS beads showed a narrow range of fluorescence. After infection with HHV-6, two transcripts of the immediate early genes were detected with both cell populations. HHV-6 suppressed colony formation of BFU-E, CFU-GM, and CFU-Meg. HHV-6 suppressed cell growth after 3 to 7 days culture in the presence of thrombopoietin (TPO). More differentiated CD34+ cells were more susceptible to the effects of HHV-6. These data indicate that the targets for hematopoietic suppression by HHV-6 are the differentiated cells.
| Original language | English |
|---|---|
| Pages (from-to) | 444-450 |
| Number of pages | 7 |
| Journal | Journal of Medical Virology |
| Volume | 70 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jul 1 2003 |
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Keywords
- Beta herpesvirinae
- Colony formation of erythroid (BFU-E)
- Granulocyte/macrophage (CFU-GM)
- Megakaryocyte (CFU-Meg)
ASJC Scopus subject areas
- Virology
Cite this
Interaction of human herpesvirus 6 with human CD34 positive cells. / Isomura, Hiroki; Yoshida, Mariko; Nanba, Hikaru; Yamada, Masao.
In: Journal of Medical Virology, Vol. 70, No. 3, 01.07.2003, p. 444-450.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interaction of human herpesvirus 6 with human CD34 positive cells
AU - Isomura, Hiroki
AU - Yoshida, Mariko
AU - Nanba, Hikaru
AU - Yamada, Masao
PY - 2003/7/1
Y1 - 2003/7/1
N2 - We reported previously that human herpesvirus 6 (HHV-6) suppresses hematopoietic colony formation of erythroid (BFU-E), granulocyte/macrophage (CFU-GM), and megakaryocyte (CFU-Meg) lineages in vitro. Here we describe the interaction between HHV-6 and human CD34+ cells, which are a major source of hematopoietic progenitor cells. CD34+ cells were immunomagnetically isolated from cord blood mononuclear cells using anti-CD34+ antibodies coated onto either Dynabeads™ or MACS beads. The CD34+ population selected with Dynabeads showed a broad range of fluorescence. The population selected with MACS beads showed a narrow range of fluorescence. After infection with HHV-6, two transcripts of the immediate early genes were detected with both cell populations. HHV-6 suppressed colony formation of BFU-E, CFU-GM, and CFU-Meg. HHV-6 suppressed cell growth after 3 to 7 days culture in the presence of thrombopoietin (TPO). More differentiated CD34+ cells were more susceptible to the effects of HHV-6. These data indicate that the targets for hematopoietic suppression by HHV-6 are the differentiated cells.
AB - We reported previously that human herpesvirus 6 (HHV-6) suppresses hematopoietic colony formation of erythroid (BFU-E), granulocyte/macrophage (CFU-GM), and megakaryocyte (CFU-Meg) lineages in vitro. Here we describe the interaction between HHV-6 and human CD34+ cells, which are a major source of hematopoietic progenitor cells. CD34+ cells were immunomagnetically isolated from cord blood mononuclear cells using anti-CD34+ antibodies coated onto either Dynabeads™ or MACS beads. The CD34+ population selected with Dynabeads showed a broad range of fluorescence. The population selected with MACS beads showed a narrow range of fluorescence. After infection with HHV-6, two transcripts of the immediate early genes were detected with both cell populations. HHV-6 suppressed colony formation of BFU-E, CFU-GM, and CFU-Meg. HHV-6 suppressed cell growth after 3 to 7 days culture in the presence of thrombopoietin (TPO). More differentiated CD34+ cells were more susceptible to the effects of HHV-6. These data indicate that the targets for hematopoietic suppression by HHV-6 are the differentiated cells.
KW - Beta herpesvirinae
KW - Colony formation of erythroid (BFU-E)
KW - Granulocyte/macrophage (CFU-GM)
KW - Megakaryocyte (CFU-Meg)
UR - http://www.scopus.com/inward/record.url?scp=0038700552&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038700552&partnerID=8YFLogxK
U2 - 10.1002/jmv.10415
DO - 10.1002/jmv.10415
M3 - Article
C2 - 12767009
AN - SCOPUS:0038700552
VL - 70
SP - 444
EP - 450
JO - Journal of Medical Virology
JF - Journal of Medical Virology
SN - 0146-6615
IS - 3
ER -