Abstract
We reported previously that human herpesvirus 6 (HHV-6) suppresses hematopoietic colony formation of erythroid (BFU-E), granulocyte/macrophage (CFU-GM), and megakaryocyte (CFU-Meg) lineages in vitro. Here we describe the interaction between HHV-6 and human CD34+ cells, which are a major source of hematopoietic progenitor cells. CD34+ cells were immunomagnetically isolated from cord blood mononuclear cells using anti-CD34+ antibodies coated onto either Dynabeads™ or MACS beads. The CD34+ population selected with Dynabeads showed a broad range of fluorescence. The population selected with MACS beads showed a narrow range of fluorescence. After infection with HHV-6, two transcripts of the immediate early genes were detected with both cell populations. HHV-6 suppressed colony formation of BFU-E, CFU-GM, and CFU-Meg. HHV-6 suppressed cell growth after 3 to 7 days culture in the presence of thrombopoietin (TPO). More differentiated CD34+ cells were more susceptible to the effects of HHV-6. These data indicate that the targets for hematopoietic suppression by HHV-6 are the differentiated cells.
Original language | English |
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Pages (from-to) | 444-450 |
Number of pages | 7 |
Journal | Journal of Medical Virology |
Volume | 70 |
Issue number | 3 |
DOIs | |
Publication status | Published - Jul 1 2003 |
Keywords
- Beta herpesvirinae
- Colony formation of erythroid (BFU-E)
- Granulocyte/macrophage (CFU-GM)
- Megakaryocyte (CFU-Meg)
ASJC Scopus subject areas
- Virology
- Infectious Diseases