Interaction of human herpesvirus 6 with human CD34 positive cells

Hiroki Isomura, Mariko Yoshida, Hikaru Namba, Masao Yamada

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We reported previously that human herpesvirus 6 (HHV-6) suppresses hematopoietic colony formation of erythroid (BFU-E), granulocyte/macrophage (CFU-GM), and megakaryocyte (CFU-Meg) lineages in vitro. Here we describe the interaction between HHV-6 and human CD34+ cells, which are a major source of hematopoietic progenitor cells. CD34+ cells were immunomagnetically isolated from cord blood mononuclear cells using anti-CD34+ antibodies coated onto either Dynabeads™ or MACS beads. The CD34+ population selected with Dynabeads showed a broad range of fluorescence. The population selected with MACS beads showed a narrow range of fluorescence. After infection with HHV-6, two transcripts of the immediate early genes were detected with both cell populations. HHV-6 suppressed colony formation of BFU-E, CFU-GM, and CFU-Meg. HHV-6 suppressed cell growth after 3 to 7 days culture in the presence of thrombopoietin (TPO). More differentiated CD34+ cells were more susceptible to the effects of HHV-6. These data indicate that the targets for hematopoietic suppression by HHV-6 are the differentiated cells.

Original languageEnglish
Pages (from-to)444-450
Number of pages7
JournalJournal of Medical Virology
Volume70
Issue number3
DOIs
Publication statusPublished - Jul 1 2003

Keywords

  • Beta herpesvirinae
  • Colony formation of erythroid (BFU-E)
  • Granulocyte/macrophage (CFU-GM)
  • Megakaryocyte (CFU-Meg)

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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